基于非靶向代谢组学的芦笋代谢物差异分析  

Analysis of Different Metabolites in Asparagus by Non-targeted Metabolomics

作　　者：牛晓雪 包艳存 雷霆 李付军[1] 李霞[1] 牟萌[1] 路远 李保华[1] NIU Xiaoxue;BAO Yancun;LEI Ting;LI Fujun;LI Xia;MU Meng;LU Yuan;LI Baohua(Asparagus Research Institute,Weifang Academy of Agricultural Sciences,Weifang 261071,Shandong,China)

机构地区：[1]潍坊市农业科学院芦笋研究所,山东潍坊261071

出　　处：《中国蔬菜》2025年第2期71-81,共11页China Vegetables

基　　金：潍坊市现代种业科研创新团队项目;山东省重点研发计划(农业良种工程)资助项目(2022LZGC024,2024LZGC032);潍坊市科技发展计划项目(2019ZJ1141);潍坊高新区科技惠民计划项目(2020KJHM14)。

摘　　要：以鲁芦笋7号为试材,采用超高效液相色谱串联质谱技术的广泛靶向代谢组学对不同性别、颜色芦笋中的代谢物进行测定分析。结果表明:多元统计方法PCA和OPLS-DA可实现对绿芦笋雌株、绿芦笋雄株和白芦笋3个样本的明显区分;对检测鉴定到926个代谢物进行分类,可划分为12类,其中黄酮和酚酸类数量最多,占代谢物总数量的35.6%。对差异代谢物进行筛选分析,发现绿芦笋雄株内甾体含量高于雌株,在鉴定到的39种甾体类代谢物中有31种含量升高;绿芦笋雌株和雄株中大部分甾体皂苷含量低于白芦笋,降低的甾体类数量约占甾体总数量的84.6%,黄酮和酚酸类含量大部分高于白芦笋,升高的数量分别约占各自类别总数量的62.4%、45.5%。对不同性别、颜色芦笋的差异代谢物进行功能注释、聚类分析,分别找到了4种和7种差异倍数较大的代谢物,其中属于甾体类的薯蓣皂苷元为绿芦笋雌株和雄株特有的差异代谢物,松柏苷、槲皮素、3-磷酸-D-甘油酸、1-O-芥子酰-D-葡萄糖、乙酸胍为绿芦笋和白芦笋特有的差异代谢物。研究结果为芦笋育种、品质分析及保健功能的挖掘提供理论依据。The metabolites in asparagus with different gender and color were determined and analyzed by extensive targeted metabolomics based on Ultra-high performance liquid chromatography-tandem mass spectrometry with‘Lulusun No.7'as the material.The results showed that samples of GAF,GAM and WA Were distinguished by PCA and OPLS-DA analysis.A total of 926 metabolites identified by detection were classified into 12 categories,among which flavonoids and phenolic acids were the most abundant,accounting for 35.6%of the total number of metabolites.Differential metabolites were screened and analyzed.It was found that contents of steroids in GAM were higher than that in GAF,and the contents of 31 out of 39 identified steroid metabolites were increased.The contents of steroidal saponins in GAM and GAF were lower than those in WA,and the number of down-regulated steroids accounted for about 84.6%of the total steroids.The contents of flavonoids and phenolic acids were higher than those in white asparagus,and the number of up-regulated accounted for about 62.4%and 45.5%of the total,respectively.Functional annotation and cluster analysis were carried out on the different metabolites in different gender and color asparagus,and 4 and 7 kinds of metabolites with larger difference multiple were found respectively.Diosgenin belonging to the steroid class was the unique differential metabolite of female and male asparagus.Coniferin,quercetin,3-phospho-D-glyceric acid,1-O-sinapyl-D-glucose and guanidinoacetate were unique differential metabolites of green and white asparagus.The results provide a theoretical basis for breeding,quality analysis and exploration of health functions in asparagus.

关 键 词：芦笋 性别 颜色 代谢物 

分 类 号：S644.6[农业科学—蔬菜学]