广西生姜青枯病菌的遗传多样性与致病力分化  

Genetic Diversity and Pathogenicity Differentiation of Ralstonia solanacearum Isolated from Ginger in Guangxi

作　　者：杨维超 李小莉 黄皓[2] 陈媛媛 文俊丽[2] 周生茂[2] 袁高庆[1] YANG Weichao;LI Xiaoli;HUANG Hao;CHEN Yuanyuan;WEN Junli;ZHOU Shengmao;YUAN Gaoqing(College of Agriculture,Guangxi University,Nanning 530004,Guangxi,China;Vegetable Research Institute of Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;College of Forestry Engineering,Guangxi Ecoengineering Vocational and Technical College,Liuzhou 545003,Guangxi,China)

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西农业科学院蔬菜研究所,广西南宁530007 [3]广西生态工程职业技术学院林业工程学院,广西柳州545003

出　　处：《中国蔬菜》2025年第2期140-147,共8页China Vegetables

基　　金：广西重点研发计划项目(桂科AB21238002);广西农业科学院基本科研业务专项(2015YZ03,桂农科2024YP082)。

摘　　要：生姜青枯病由青枯雷尔氏菌(Ralstonia solanacearum)引起,是广西生姜产区重要的土传病害。为了明确广西生姜青枯病菌的遗传多样性及致病力分化,收集菌株,基于该病菌演化型分类框架和egl基因序列进行系统发育分析,同时刺伤生姜植株茎基部后灌施菌液进行接种,对病情指数进行聚类分析。结果显示,广西生姜主产区的42株青枯病菌菌株均为演化型Ⅰ型,分属于序列变种14、16和30,以前两种为主。通过致病力测定,供试菌株可划分为3类:64.29%的菌株表现为强致病力,病情指数为83.70~100.00;21.43%的菌株表现为较强致病力,病情指数为62.22~75.56;仅有14.29%的菌株表现为较弱至中等致病力,病情指数为24.44~51.11。表明广西生姜青枯病菌种群存在一定的遗传多样性和明显的致病力分化,为开展其致病机制研究和生姜抗青枯病育种提供重要参考。Ginger bacterial wilt is caused by Ralstonia solanacearum,which is an important soil-borne disease in the ginger production area of Guangxi.In order to clarify the genetic diversity and pathogenicity differentiation of R.solanacearum on ginger in Guangxi,this study collected strains,and conducted phylogenetic analysis based on the phylotype classification framework and egl gene sequence of the pathogen.At the same time,R.solanacearum strains were inoculated by irrigating the bacterial suspension after stabbing the stem base of ginger plant,and cluster analysis was conducted on the disease index.The results showed that all 42 strains of R.solanacearum isolated from main ginger production areas of Guangxi were identified as phylotype I,belonging to sequevar 14,16 and 30,with the former two being the main types.Through pathogenicity testing,the strains were divided into three categories:64.29%of the strains showed very strong pathogenicity,with the disease index of 83.70 to 100.00;21.43%of the strains showed strong pathogenicity,with the disease index of 62.22 to 75.56;Only 14.29%of the strains showed weak to moderate pathogenicity,with the disease index of 24.44 to 51.11.These results indicated that there is a certain degree of genetic diversity and significant pathogenicity differentiation in the population of R.solanacearum on ginger in Guangxi,providing important references for researching their pathogenic mechanisms and breeding for resistance to the disease.

关 键 词：生姜 青枯雷尔氏菌 遗传多样性 致病力分化 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]