等渗NaCl和PEG6000对中亚沙棘种子萌发和幼苗生长的影响  

Effect of Iso-osmotic NaCl and PEG6000 Stress on Seed Germination and Seedling Growth of Hippophae rhamnoides subsp.turkestanica

作　　者：庄重 张玲[1,2] 邱爱军 刘艳萍[1,2] 周美 ZHUANG Zhong;ZHANG Ling;QIU Aijun;LIU Yanping;ZHOU Mei(Xinjiang Production&Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Alaer,Xinjiang Uygur Autonomous Region 843300,China;College of Life Science and Technology,Tarim University,Alaer,Xinjiang Uygur Autonomous Region 843300,China;College of Chemical and Technology,Tianshui Normal University,Tianshui,Gansu 741000,China)

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆维吾尔自治区阿拉尔843300 [2]新疆塔里木大学生命科学与技术学院,新疆维吾尔自治区阿拉尔843300 [3]天水师范学院化学工程与技术学院,甘肃天水741000

出　　处：《林业与环境科学》2025年第1期70-76,共7页Forestry and Environmental Science

基　　金：国家自然科学基金(31660048)。

摘　　要：为探讨盐、干旱胁迫对中亚沙棘Hippophae rhamnoides subsp.turkestanica种子萌发和幼苗生长的影响,该研究采用等渗NaCl和PEG6000溶液模拟盐和干旱胁迫,以清水处理为对照组(CK),设置-0.2、-0.4、-0.6、-0.8和-1.0 MPa共5个渗透势水平,每日观察并记录中亚沙棘种子萌发和幼苗生长状况。结果表明,中亚沙棘种子最终萌发率随PEG溶液渗透势降低而降低,而NaCl溶液对其没有显著影响;NaCl和PEG胁迫下中亚沙棘的最终萌发率分别为96.7%~100%和0~97.5%,NaCl对其萌发影响不显著,而PEG影响显著(P<0.05);PEG处理组复水后萌发率达100%,表明干旱胁迫可导致种子休眠,但种子的活力仍能保持。NaCl和PEG胁迫延后了其初始萌发时间并延长萌发周期,且PEG胁迫的抑制作用较NaCl胁迫剧烈。通过对中亚沙棘幼苗的鲜质量和苗高统计分析,发现NaCl处理组的中亚沙棘幼苗鲜质量先升高后降低,PEG处理组鲜质量明显降低;NaCl和PEG对幼苗苗高有抑制作用,处理组苗高均低于CK,且NaCl胁迫主要表现在根伸长生长受到抑制,PEG胁迫组根短且弯曲。PEG对中亚沙棘种子萌发和幼苗生长的抑制程度强于等渗NaCl,说明在盐和干旱胁迫条件下,渗透胁迫是影响其萌发的主要因素,中亚沙棘对干旱胁迫较盐胁迫更敏感,同时对盐胁迫具有一定耐受性。To investigate the effects of salt and drought stress on seed germination and seedling growth of Hippophae rhamnoides subsp.turkestanica,iso-osmotic NaCl and PEG6000 solutions were used to simulate salt and drought stress,respectively,and water treatment was used as the control group(CK).Five osmotic potential levels of-0.2,-0.4,-0.6,-0.8,and-1.0 MPa were set up,and the seed germination and seedling growth of H.rhamnoides subsp.turkestanica was observed and recorded daily.The results showed that the seed final germination rate decreased with the decrease in the osmotic potential of PEG solution,while NaCl solution had no significant effect.The final germination rates of H.rhamnoides subsp.turkestanica under NaCl and PEG stress were 96.7%-100%and 0-97.5%,respectively.NaCl had no significant effect on its germination,while PEG had a significant effect(P<0.05).The germination rate of the PEG treatment group was 100%after rehydration,indicating that drought stress could lead to seed dormancy,but seed vigor could still be maintained.NaCl and PEG stress delayed the initial germination time and prolonged the germination cycle,with the inhibitory effect of iso-osmotic PEG stress being stronger than that of NaCl stress.Through the statistical analysis of the fresh weight and seedling height of seabuckthorn seedlings,it was found that the seedling fresh weight in NaCl treatment group increased first and then decreased,and the fresh weight of iso-osmotic PEG treatment group decreased significantly.NaCl and PEG inhibited the seedling height,and the seedling height of the treatment group was lower than that of CK,and NaCl stress was mainly manifested in the inhibition of root elongation growth,and the root of PEG stress group was short and curved.The inhibition of iso-osmotic PEG on seed germination and seedling growth of H.rhamnoides subsp.turkestanica was stronger than that of isotonic NaCl,indicating that osmotic stress was the main factor affecting its germination under salt and drought stress conditions.H.rhamnoides subs

关 键 词：盐胁迫 PEG胁迫 复水 种子萌发 幼苗生长 中亚沙棘 

分 类 号：S793.6[农业科学—林木遗传育种]