番鸭腺病毒的分离鉴定及hexon基因遗传变异研究  

Identification and Hexon Gene Variation of Muscovy Duck Adenovirus

作　　者：林锋强[1] 陈小丽 李典辉 沈华伟 董慧 朱小丽[1] 王劭[1] LIN Fengqiang;CHEN Xiaoli;LI Dianhui;SHEN Huawei;DONG Hui;ZHU Xiaoli;WANG Shao(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fuzhou,Fujian 350013,China;Sanming Bureau of Agriculture and Rural Affairs,Sanming,Fujian 365000,China;Provincial Animal Husbandry Station,Fuzhou,Fujian 350000,China)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013 [2]三明市农业农村局,福建三明365000 [3]福建省畜牧总站,福建福州350000

出　　处：《福建农业学报》2024年第12期1346-1353,共8页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划公益类专项(2024R1025007);福建省星火计划项目(2022S0011)。

摘　　要：【目的】研究福建省番鸭白肝病病原特性、流行病学特征及病毒遗传变异状况。【方法】从福建省福州、三明、莆田、漳州等地共收集临床疑似番鸭白肝病例9份,进行鸭腺病毒(duck adenovirus,DAdV)病原学检测,包括病原分离鉴定、血凝特性、致病性分析及hexon基因序列分析。【结果】鸭腺病毒感染主要在春秋两季发生;发病率15%~40%,死亡率5%~15%。采集病死鸭病料,检测到鸭腺病毒感染;利用番鸭胚进行病毒分离,分离毒均能致死番鸭胚。番鸭感染分离毒后发病率为60%~100%,死亡率为20%~40%。9株DAdV分离毒hexon基因核苷酸同源性在99.9%以上,序列特征与CH-GD-12-2014株一致,未发生碱基缺失、插入或基因重组,较为稳定;与法国分离株DAdV-GR株同源性在76.6%~76.7%;与禽腺病毒(fowl adenovirus,FAdV)同源性在64.1%~66.8%。进化树分析显示9株分离毒与GD-12-2014株和国外分离株GR株属于同一分支,而与FAdV属于不同分支。【结论】2022年福建省流行的番鸭腺病毒毒株为2型腺病毒。本研究结果为深入研究鸭2型腺病毒致病机理及其疫苗研制和综合防控措施提供了重要的临床资料和基础数据。【Objective】Characteristics and epidemiology of the muscovy duck white hepatitis in Fujian were studied,and genetic variations of the pathogen capsid protein determined.【Methods】Clinical specimens of 9 suspected muscovy duck white hepatitis cases incurred at Fuzhou,Sanming,Putian,and Zhangzhou in the province were collected to test for DAdVs using pathogen detection methods.Isolated pathogens were identified,and their hemagglutination characteristics,pathogenicity,and hexon gene sequences determined.【Results】The disease was found on muscovy ducks mostly in spring and autumn with an incidence rate of 15%–40%and a mortality rate of 5%–15%.The DAdVs were isolated and tested to be lethal to muscovy duck embryos.The infection rate of the virus on ducks ranged 60%–100%with a mortality rate of 20%–40%.The homology of the hexon gene nucleotides among the 9 isolated DAdVs was greater than 99.9%.The capsid proteins had sequences similar to that of CH-GD-12-2014 and were stable proteins without base deletion,insertion or recombination.The 9 isolates had a 99.9%–100%homology with CH-GD-12-2014 from Guangdong,76.6%–76.7%with DAdV-GR from France,and 64.1%–66.8%with FAdV and other serotypes.They were in the same branch with GD-12-2014 and GR,but not AdV,on the phylogenetic tree.【Conclusion】The strains of the muscovy duck adenovirus circulating in Fujian Province in 2022 belonged to the DAdV Type 2.

关 键 词：番鸭腺病毒 流行病学 病原 hexon基因 

分 类 号：S851.3[农业科学—预防兽医学]