干旱胁迫下锥栗幼苗的代谢组学分析  

Metabolomic Analysis of Castanea henryi Seedling under Drought Stress

作　　者：童琪 任少华[1] 张梅 张驰强 王陈 李贵[2] 陈瑞[2] 吴敏[2] 李媛媛[1] LI Gui;CHEN Rui;WU Min;LI Yuanyuan(Guizhou Botanical Garden,Guiyang Guizhou 550004,P.R.China;Hunan Academy of Forestry,Changsha Hunan 410004,P.R China)

机构地区：[1]贵州省植物园,贵州贵阳550004 [2]湖南省林业科学院,湖南长沙410004

出　　处：《西部林业科学》2025年第1期58-66,共9页Journal of West China Forestry Science

基　　金：贵州省植物园园级科技项目(黔植科合〔2023〕05号);贵州省自然科学基金项目(黔科合基础-ZK〔2022〕一般291号)。

摘　　要：锥栗是我国南方重要的木本粮食树种。为探究其响应不同程度干旱胁迫的分子机制,以锥栗幼苗为对象,设置对照组(土壤相对含水量80%~90%)以及干旱胁迫T2(土壤相对含水量40%~50%)和T4(土壤相对含水量20%~30%)两个处理组,采集叶片进行代谢组学分析。结果显示:CK vs T2处理的差异代谢物总数为128个,CK vs T4处理的差异代谢物总数为124个,T2 vs T4处理的差异代谢物总数为164个。在干旱胁迫10 d和20 d条件下共有极显著上调(P<0.01)的差异代谢物为衣康酸,判定其是锥栗抗干旱的关键代谢物质。通过代谢通路的影响因子总共筛选出14条主要通路,其中β-丙氨酸代谢与淀粉和蔗糖代谢是干旱胁迫10 d和20 d条件下共同存在的代谢通路,证明这2条代谢通路与锥栗抗旱性存在密切关联。研究结果可为深入研究锥栗的耐旱机制奠定理论基础。Castanea henryi is an important woody grain tree species in southern China,and the molecular mechanism of its response to different degrees of drought requires further investigation.In this study,we utilized C.henryi as our research subject,establishing a control group(soil relative water content 80%-90%)and two treatment groups of drought stress T2(soil relative water content 40%-50%)and T4(soil relative water content 20%-30%).Leaf samples of C.henryi were collected for metabolomics analysis.There were 128 differential metabolites at CK vs T2 groups.There were 124 differential metabolites at CK vs T4 groups.There were 164 differential metabolites at T2 vs T4 groups.The significantly upregulated(P<0.01)differential metabolite under drought stress conditions of ten and twenty days was itaconic acid,which was identified as a key metabolite for drought resistance in C.henryi.Fourteen major pathways were screened based on the influence factors of metabolic pathways.Among these pathways,starch and sucrose metabolism and beta-Alanine metabolism were co-coexisting metabolic pathways under drought stress conditions of ten and twenty days.It could be proven that the two metabolic pathways were associated with C.henryi drought resistance.These results provided a theoretical foundation for further studies aimed at drought tolerance mechanism in C.henryi.

关 键 词：锥栗 干旱胁迫 代谢组 差异代谢物 

分 类 号：S664.2[农业科学—果树学]