大花序桉木材力学性质相关的基因表达及可变剪接分析  

Analysis of Gene Expression and Alternative Splicing Related to Wood Mechanical Properties of Eucalyptus cloeziana

作　　者：陈升侃 黄瑞琛 李建凡 孙熹 黄星波 邓紫宇[1] 卢翠香[1] 李昌荣[1] CHEN Shengkan;HUANG Ruichen;LI Jianfan;SUN Xi;HUANG Xingbo;DEND Ziyu;LU Cuixiang;LI Changrong(Key Laboratory of Central South Fast-growing Timber Cultivation of National Forestry and Grassland Administration,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation/Guangxi Forestry Research Institute,Nanning Guangxi 530002,P.R.China;Yulin Forestry Research Institute,Yulin Guangxi 537501,P.R.China;Guangxi Nanning arboretum,Nanning Guangxi 530025,P.R.China)

机构地区：[1]广西壮族自治区林业科学研究院国家林业和草原局中南速生材繁育实验室/广西优良用材林资源培育重点实验室,广西南宁530002 [2]玉林市林业科学研究所,广西玉林537501 [3]广西壮族自治区南宁树木园,广西南宁530025

出　　处：《西部林业科学》2025年第1期101-111,共11页Journal of West China Forestry Science

基　　金：广西优良用材林资源培育重点实验室课题(2020-A-01-01);广西林业科技项目(桂林科研[2021]5号)。

摘　　要：为加深对木材力学性质基因调控的了解,促进大花序桉的遗传改良,以大花序桉群体为材料,根据群体的木材力学性质(包括基本密度、抗弯弹性模量、抗弯强度和顺纹抗压强度)建立极值基因池,并对其形成层进行转录组测序,研究大花序桉木材力学性质相关的差异表达基因和差异剪接基因。结果显示:转录组测序数据经质控后,平均获得高质量reads数42227420条,平均GC含量51.72%,与参考基因组的比对率平均为69.62%。基本密度、抗弯弹性模量、抗弯强度和顺纹抗压强度比较组分别筛选出3983、2774、3038个和1771个显著差异表达基因,其中181个为核心差异表达基因,可能通过调控木质素的含量和单体比例、次生细胞壁厚度和强度、网络结构修饰、细胞伸长、微纤丝角和纤维素结晶状态等,影响大花序桉的木材力学性质。大花序桉形成层检测到可变剪接事件5302~5894个,最主要的可变剪接形式是外显子跳跃,占38.5%~40.7%,基本密度、抗弯弹性模量、抗弯强度和抗压强度的极值比较组中分别筛选出差异剪接基因63、100、180个和75个,表明可变剪接也是调控木材力学性质的重要因素。差异剪接基因与差异表达基因重叠较少,转录组和mRNA剪接的调控相对独立。In this study,we established the extreme gene pool based on the wood mechanical properties of Eucalyptus cloeziana,including basic density(BD),modulus of elasticity(MOE),modulus of rupture(MOR)and compressive strength(CS),and and transcriptome sequencing was performed on the cambium.The different expressed genes(DEGs)and spliced genes(DSGs)related to wood mechanical properties of E.cloeziana were identified,aiming to deepen the understanding of gene regulation of wood mechanical properties and promote the genetic improvement of E.cloeziana.The results showed that,after quality control of transcriptome sequencing data,an average of 42227420 high-quality reads were obtained,with an average GC content of 51.72%and an average alignment rate with the reference genome of 69.62%.A total of 3983,2774,3038 and 1771 significantly DEGs were screened out in the comparison groups of BD,MOE,MOR and CS,respectively,of which 181 were core DEGs.These core DEGs may affect the wood mechanical properties by regulating lignin content and monomer ratio,secondary cell wall thickness and strength,network structure modification,cell elongation,microfibril angle and cellulose crystallization state.A total of 5302-5894 alternative splicing events were detected in E.cloeziana cambium,and the most important alternative splicing form was exon skipping,accounting for 38.5%-40.7%.There were 63100180 and 75 DSGs screened in the BD,MOE,MOR,and CS,respectively.These results indicate that alternative splicing is also an important factor in the regulation of wood mechanical properties.There is little overlap between DSGs and DEGs,indicating the regulation of transcriptome and mRNA splicing is relatively independent.

关 键 词：大花序桉 木材力学性质 转录组 差异表达基因 可变剪接 

分 类 号：S722.3[农业科学—林木遗传育种]