细胞周期蛋白M4的纯化和初步预测结构探讨  

Investigation of purification and preliminary predicted structure of CNNM4

作　　者：马晨雨 强亦龙 黄天云 石欣雨 廖丹 解媛 林伟[1] MA Chenyu;QIANG Yilong;HUANG Tianyun;SHI Xinyu;LIAO Dan;XIE Yuan;LIN Wei(Department of Neurosurgery,Xijing Hospital,Fourth Military Medical University,Xi’an,Shaanxi 710032,China;Graduate Department,Xi’an Medical University,Xi’an,Shaanxi 710032,China;Department of Pharmacy,Xi’an No.3 Hospital,the Affiliated Hospital of Northwest University,Xi’an,Shaanxi 710032,China)

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032 [2]西安医学院研究生工作部,陕西西安710021 [3]西北大学附属医院西安市第三医院药剂科,陕西西安710021

出　　处：《中华神经外科疾病研究杂志》2025年第2期7-11,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金(32200803);中国人民解放军空军军医大学第一附属医院A层次博士科研启动项目(XJARC05);陕西省自然科学基础研究计划重点项目(2021JZ-34)。

摘　　要：目的对细胞周期蛋白M4(CNNM4)进行表达和纯化,并对其预测结构进行初步探讨。方法利用Bac to Bac哺乳动物表达系统进行蛋白表达,通过质粒设计、质粒扩增、杆粒制备、细胞转染、病毒制备、蛋白表达及蛋白纯化分析等工作对CNNM4进行蛋白纯化,并获得纯度和均一性良好的蛋白质样品。结果成功获得了纯度和均一性良好的CNNM4蛋白质样品。结论CNNM4成功表达、纯化,并对其预测结构进行了初步探讨。Objective Discussion of expression and purification,and the predicted structure of the cyclin M4 protein(CNNM4).Methods The Bac to Bac mammalian expression system was used for protein expression.Plasmid design,plasmid amplification,bacmid preparation,cell transfection,virus preparation,protein expression and protein purification of CNNM4 were performed so as to obtain protein samples with good purity and uniformity.Results CNNM4 protein samples with good purity and uniformity were obtained successfully.Conclusions CNNM4 is successfully expressed and purified,and its predicted structure is preliminarily discussed.

关 键 词：蛋白质表达纯化 细胞周期蛋白M4 镁离子 

分 类 号：Q7[生物学—分子生物学]