聚乙烯亚胺介导Bmk CT基因抑制C6胶质瘤细胞VEGF、MMP-9、AQP-1表达  

Polyethyleneimine-mediated Bmk CT gene inhibition of VEGF,MMP-9,AQP-1 expressions in C6 glioma cells

作　　者：张亚涛 胡文涛 蓝金星 蔡玲 徐东刚[3] 刘嘉霖[2] ZHANG Yatao;HU Wentao;LAN Jinxing;CAI Ling;XU Donggang;LIU Jialin(Department of Intensive Care Medicine,South Courtyard Branch,West Institute Hospital of Luoyang Hospital of Traditional Chinese Medicine,Luoyang,Henan 471000,China;Department of Neurosurgery,First Medical Center,PLA General Hospital,Beijing 100853,China;Academy of Military Medical Sciences,Beijing 100853,China)

机构地区：[1]洛阳市中医院西工院区南院重症医学科,河南洛阳471000 [2]解放军总医院第一医学中心神经外科医学部,北京100853 [3]军事医学科学院,北京100853

出　　处：《中华神经外科疾病研究杂志》2025年第2期12-16,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金(82473264,82172680,82373220)。

摘　　要：目的东亚钳蝎氯离子通道毒素(Bmk CT)基因与非病毒载体阳离子聚合物聚乙烯亚胺(PEI)结合转染C6胶质瘤细胞,观察其对C6细胞中VEGF、MMP-9、AQP-1、p-ERK、p-AKT、p-NF-κBp65表达的影响。方法通过生物信息学分析VEGF、MMP-9、AQP-1与胶质瘤恶性进展的关系。将PEI与空质粒pEGFP-N1和基因质粒pEGFP-N1-Bmk CT分别混合。将C6细胞分别转染PEI/pEGFP-N1和PEI/pEGFP-N1-BMKCT两种复合体。24 h后使用荧光显微镜观察转染情况。48 h后,用Western-blot检测VEGF、MMP-9、AQP-1、p-ERK、p-AKT、p-NF-κBp65蛋白表达水平。结果VEGF、MMP-9、AQP-1与胶质瘤恶性进展密切相关。24 h后观察转染成功。与PEI/pEGFP-N1组相比,PEI/pEGFP-N1-Bmk CT组在转染48 h后VEGF、MMP-9、AQP-1、p-ERK、p-AKT、p-NF-κBp65蛋白表达水平下降。结论使用PEI/pEGFP-N1-Bmk CT复合物转染C6细胞能抑制VEGF、MMP-9、AQP-1表达,减轻肿瘤相关性水肿,这可能与ERK、AKT/NF-κB通路受抑制有关。Objective To investigate the effects of polyethyleneimine(PEI)-mediated Buthus martensii Karsch Chlorotoxin-like Toxin(Bmk CT),and Bmk CT gene transfection on the expression levels of VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65 of C6 glioma cells.Methods pEGFP-N1 and pEGFP-N1-BmK CT were respectively combined with PEI to form two complexes which were used to transfect C6 cells.After 48 hours’test by Western bloting,VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65 were detected.Results The expression levels of VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65 in the pEGFP-N1-Bmk CT group were significantly lower than those of the pEGFP-N1 group after the transfection.Conclusions PEI/pEGFP-N1-Bmk CT may suppress tumor associated edema through inhibiting the expressions of VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65.

关 键 词：Bmk CT 胶质瘤 VEGF MMP-9 AQP-1 通路 

分 类 号：Q78[生物学—分子生物学]