鼠尾草酚改善糖尿病小鼠认知功能障碍的机制  

Mechanism on the Improvement of Cognitive Dysfunction in Diabetic Mice by Carnosol

作　　者：张强 单伟 王刚 ZHANG Qiang;SHAN Wei;WANG Gang(The First Affiliated Hospital of Jinzhou Medical University;Human Anatomy Section,School of Basic Medicine,Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学附属第一医院 [2]锦州医科大学基础医学院人体解剖学教研室,辽宁锦州121000

出　　处：《锦州医科大学学报》2025年第1期10-15,共6页Journal of Jinzhou Medical University

基　　金：辽宁省教育厅科学研究项目,项目编号:JYTJCZR2020087。

摘　　要：目的探讨鼠尾草酚对糖尿病小鼠认知功能的改善情况。方法腹腔注射链脲佐菌素构建小鼠糖尿病模型,干预组小鼠给予100 mg/kg鼠尾草酚灌胃。记录小鼠造模前、造模3 d和12周的体重和血糖;Morris水迷宫评估糖尿病小鼠的认知功能;Nissl染色观察海马组织神经元形态学及数量的改变;ELISA检测小鼠脑组织中的超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛(malondialdehyde,MDA)含量;Western Blot检测海马CX3CL1、CX3CR1蛋白及氧化应激超氧化物歧化酶(superoxide dismutase 2,SOD2)的蛋白表达水平。结果血糖和体重结果显示,与对照组(CON组)相比,在12周时糖尿病相关认知障碍组(DM组)血糖明显升高,体重减轻(P<0.05),而鼠尾草酚(carnosol,CL)干预后改善上述变化(P<0.05)。Morris水迷宫实验结果显示,与DM组相比,CL组小鼠逃避潜伏期明显缩短,穿越平台次数明显增加(P<0.05)。Nissl染色发现CL干预后Nissl体数量有所增加,神经元形态明显改变。与DM组相比,CL治疗组小鼠脑组织中SOD活力增加(P<0.05),MDA含量降低(P<0.05)。CX3CL1、CX3CR1蛋白表达均上调(P<0.05),SOD2明显上调(P<0.05)。结论鼠尾草酚可能通过CX3CL1/CX3CR1通路缓解DM小鼠海马组织的氧化应激,减缓神经元损伤,进而改善DM小鼠认知功能。Objective To investigate the improvement of cognitive function in diabetic mice by Carnosol.Methods Construction of a diabetes model by intraperitoneal injection of streptozotocin in mice,and mice in the intervention group were given Carnosol gavage.Body weight and blood glucose of mice before modeling,3 d and 12 weeks of modeling were recorded;Morris Water Maze was used to assess the cognitive function in Diabetic Mice;Nissl staining was used to observe changes in the morphology and the number of neurons in hippocampal tissue;ELISA was used to for SOD activity and MDA content in mouse brain tissue;Western Blot was used to detect the protein expression levels of hippocampal CX3CL1,CX3CR1 proteins and oxidative stress SOD2.Results Blood glucose and weight results showed a significant increase in blood glucose and weight loss in the DM group at 12 weeks compared to the CON group(P<0.05),while the CL intervention improved these changes(P<0.05).The results of Morris Water Maze experiments showed that mice in the CL group had a significantly shorter escape latency and a significantly higher number of traversed platforms compared to the DM group(P<0.05).Nissl staining revealed an increase in the number of Nissl bodies and a significant change in neuronal morphology after CL intervention.Compared with the DM group,SOD activity was increased(P<0.05)and MDA content was decreased(P<0.05)in the brain tissue of mice in the CL treatment group.CX3CL1 and CX3CR1 protein expression were up-regulated(P<0.05,and SOD2 was significantly up-regulated(P<0.05).Conclusion Carnosol may alleviate oxidative stress and slow down neuronal damage in hippocampal tissues of DM mice through the CX3CL1/CX3CR1 pathway,which in turn improves cognitive function in DM mice.

关 键 词：鼠尾草酚 糖尿病相关认知功能障碍 氧化损伤 CX3CL1/CX3CR1 

分 类 号：R285[医药卫生—中药学]