应用HPLC法同时检测奈玛特韦和利托那韦血药浓度  

Simultaneous Detection of Blood Drug Concentrations of Remdesivir and Ritonavir by HPLC and Their Clinical Application

作　　者：李雪 LI Xue(Department of Pharmacy,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学附属第一医院药学部,辽宁锦州121000

出　　处：《锦州医科大学学报》2025年第1期45-50,共6页Journal of Jinzhou Medical University

摘　　要：目的建立人血浆样品中奈玛特韦和利托那韦的高效液相色谱(high performance liquid chromatography,HPLC)同时检测方法。方法本实验采用高效液相色谱中的梯度洗脱法对样品进行分析,选用伊利特Hypersil BDS C18色谱柱,规格为4.6 mm×250 mm,粒径5μm。该色谱柱搭配紫外检测器,利用物质对紫外光的吸收特性检测洗脱的化合物。实验的流动相由乙腈和0.1%甲酸水组成,流速设置为0.3 mL/min,柱温保持在55℃,进样量为10μL,定量检测患者奈玛特韦、利托那韦的血药浓度。结果人血浆样品各成分在0.1~60μg/mL内线性关系良好(r>0.99),低、中、高质控血浆样品的提取回收率为86.93%~97.10%。日内、日间精密度、准确度和稳定性均符合生物样品分析要求。应用该方法检测6名患者血浆中奈玛特韦和利托那韦的药物浓度,奈玛特韦的药物浓度为0.69~0.81μg/mL,利托那韦的药物浓度为0.52~1.39μg/mL。结论建立的HPLC-UV定量分析方法快速、准确、灵敏,可用于人血浆中奈玛特韦、利托那韦的含量测定。Objective To establish a high performance liquid chromatography(HPLC)analysis method for the detection of remdesivir and ritonavir in human plasma samples.Methods In this experiment,the samples were analyzed by gradient elution method in high performance liquid chromatography.The chromatographic column of Elite Hypersil BDS C18 was selected,the size was 4.6 mm×250 mm,and the particle size was 5μm.The column was equipped with an ultraviolet detector to detect the elution compounds using the absorption properties of the substances to ultraviolet light.The mobile phase of the experiment was composed of acetonitrile and 0.1%formic acid water,the flow rate was set at 0.3 mL/min,the column temperature was maintained at 55℃,the injection volume was 10μL,and the blood concentrations of nematovir and ritonavir were quantitatively detected.Results The linear relationship of two components in human plasma samples was good within the range of 0.1-60μg/mL(r>0.99),and the extraction recovery rates of low,medium,and high quality control plasma samples were 86.93%-97.10%.Both intra-day and inter-day precision,accuracy,and stability met the requirements for biological sample analysis.This method was applied to detect the drug concentrations of remdesivir and ritonavir in the plasma of 6 patients.The drug concentration of remdesivir was 0.69-0.81μg/mL,and the drug concentration of ritonavir was 0.52-1.39μg/mL.Conclusion The HPLC-UV quantitative analysis method established is rapid,accurate,and sensitive,and can be used for the determination of the content of remdesivir and ritonavir in human plasma.

关 键 词：高效液相色谱检测法 奈玛特韦 利托那韦 

分 类 号：R969.1[医药卫生—药理学]