伴或不伴哮喘的慢性鼻窦炎伴鼻息肉患者鼻腔灌洗液蛋白质组学分析  

Proteomics comparison of nasal lavage fluid in chronic rhinosinusitis with nasal polyps with or without asthma

作　　者：罗祥煌 郭静[2] 姚尧 杨玉娟 王建伟[2] 于鹏翼 张文彬[2] 张宇[2] 宋西成[2] LUO Xianghuang;GUO Jing;YAO Yao;YANG Yujuan;WANG Jianwei;YU Pengyi;ZHANG Wenbin;ZHANG Yu;SONG Xicheng(School of Clinical Medicine,Shandong Second Medical University,Weifang,Shandong,261000,China;Department of Otolaryngology Head and Neck Surgery,Yantai Yuhuangding Hospital,Qingdao University,Shandong Provincial Clinical Research Center for Otolaryngologic Diseases,Yantai Key Laboratory of Otorhinolaryngologic Diseases,Yantai,Shandong,264000,China)

机构地区：[1]山东第二医科大学临床医学院,山东潍坊261000 [2]青岛大学附属烟台毓璜顶医院耳鼻咽喉头颈外科,山东省耳鼻喉疾病临床医学研究中心,烟台市耳鼻喉疾病重点实验室,山东烟台264000

出　　处：《中国耳鼻咽喉头颈外科》2025年第1期37-41,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金(82271146、82271147);山东省重大科技创新工程(2020CXGC011302、2022CXGC020506)。

摘　　要：目的探讨共病哮喘对慢性鼻窦炎伴鼻息肉(CRSwNP)的影响,并识别关键蛋白和信号通路。方法采用蛋白质组学方法,分析对照组、CRSwNP组和CRSwNP合并哮喘组鼻灌洗液(nasal lavage fluid,NLF)中的差异表达蛋白(differentially expressed proteins,DEPs)。通过数据独立性采集分析技术,评估三组间DEPs的梯度变化,确定共病哮喘对CRSwNP影响的关键蛋白。结果与对照组相比,CRSwNP组和CRSwNP合并哮喘组分别鉴定出1377个和1006个DEPs。上调的过氧化物酶5、Ran结合蛋白1和下调的角蛋白9被确定为影响CRSwNP合并哮喘的关键蛋白。结论共病哮喘可能通过特定关键蛋白和信号通路促进鼻息肉的发生和发展,为CRSwNP和哮喘的相互作用提供新的分子层面见解。OBJECTIVE Aimed to investigate the impact of comorbid asthma on chronic rhinosinusitis with nasal polyps(CRSwNP)and identify key proteins and signaling pathways.METHODS Proteomic methods were employed to analyze differentially expressed proteins(DEPs)in nasal lavage fluid(NLF)from control,CRSwNP,and CRSwNP with asthma groups.DIA quantitative analysis technology was used to assess the gradient changes of DEPs among the three groups to determine key proteins affected by comorbid asthma in CRSwNP.RESULTS Compared to the control group,1377 and 1006 DEPs were identified in the CRSwNP and CRSwNP with asthma groups,respectively.Peroxiredoxin-5(PRDX5),Ran-Binding Protein 1(RanBP1)(upregulated),and Keratin 9(KRT9)(downregulated)were identified as key proteins affecting CRSwNP with asthma.CONCLUSION Comorbid asthma may promote the occurrence and development of nasal polyps through specific key proteins and signaling pathways,providing new molecular insights into the interaction between CRSwNP and asthma.

关 键 词：鼻窦炎 鼻息肉 哮喘症 蛋白组学 

分 类 号：R765.41[医药卫生—耳鼻咽喉科] R765.25[医药卫生—临床医学]