羊胎盘粉对青年大鼠骨质量影响  

Effect of ovinus placenta powder on bone quality in young rats

作　　者：杨世超 宋慕格 高玉海 王龙飞 王立强 安梓栋 李亮 谢高倩 陈克明 YANG Shichao;SONG Muge;GAO Yuhai;WANG Longfei;WANG Liqiang;AN Zidong;LI Liang;XIE Gaoqian;CHEN Keming(Basic Medical Laboratory,No.940 Hospital of Joint Logistics Support Force of Chinese PLA,Lanzhou 730050,China;Clinical College of Traditional Chinese Medicine,Gansu University Of Chinese Traditional Medicine,Lanzhou 730000,China;Gansu Key Laboratory of Stem Cells and Genetic Drug,Lanzhou 730050,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院基础医学实验室,甘肃兰州730050 [2]甘肃中医药大学中医临床学院,甘肃兰州730000 [3]甘肃省干细胞与基因药物重点实验室,甘肃兰州730050

出　　处：《中国骨质疏松杂志》2025年第2期175-181,共7页Chinese Journal of Osteoporosis

基　　金：兰州市科技计划项目(2023-1-8;2023-ZD-183);甘肃省自然科学基金项目(22JR11RA013);军队后勤自主科研项目(2023HQZZ-07);联勤保障部队第九四〇医院G计划人才项目(2024-G3-10);甘肃省科技重大专项(24ZDFA008)。

摘　　要：目的探讨羊胎盘粉对青年大鼠骨质量的影响。方法选取40只SPF级一月龄SD大鼠,按照随机数字表法分为对照(Ctrl)组和羊胎盘粉低(L)、中(M)、高(H)剂量组。灌服给药6周后处死所有大鼠,进行骨密度、Micro-CT扫描、双荧光标记观察、生物力学试验、病理学检查等,通过ELISA法检测血清骨代谢指标BALP、PINP、CTX-1和TRAP水平。结果在实验过程中,各组大鼠体重、主要脏器无明显改变,脏器指数无统计学差异(P>0.05),心、肝、脾、肺、肾脏器病理学观察未见明显异常;股骨和椎骨骨密度检测结果显示,在羊胎盘粉各干预组中,与Ctrl组比较,M组和H组大鼠股骨和椎骨骨密度均显著升高;Micro-CT扫描重建结果,与Ctrl组比较,M组骨体积分数、骨表面总体积、骨小梁数量、骨小梁厚度均显著提高(P<0.05),H组极显著提高(P<0.01)。骨小梁分离度方面,M组显著下降(P<0.05),H组极显著下降(P<0.01);股骨病理学观察可见,各干预组中,骨小梁数量出现不同程度的增加,其中H组效果最为明显;骨生物力学试验结果显示,M组和H组能够显著提升大鼠股骨的最大载荷和抗压强度;双荧光标记结果显示,与Ctrl组比较,各给药组荧光标记间距均有增大,其中M组和H组较为明显;血清骨代谢指标检测结果显示,与Ctrl组比较,M组和H组能够显著提高骨形成指标PINP和BALP水平,同时H组能够显著降低CTX-1和TRAP水平(P<0.01);羊胎盘粉能够不同程度的提高成骨相关蛋白BMP2、RUNX2的表达,抑制破骨相关蛋白RANKL的表达。结论羊胎盘粉可能通过促进骨形成和抑制骨吸收来提高青年大鼠骨密度和骨强度,且在75~300 mg/kg浓度范围内呈剂量依赖性。Objective To study the effects of ovinus placenta powder on bone quality in young rats.Methods 40 SPF one-month old SD rats were selected and divided into control group and ovinus placenta powder low,medium and high dose groups according to random number table method.After 6 weeks of administration,all the rats were killed.Bone mineral density,Micro-CT scan,double fluorescence label observation,biomechanical test and pathological examination were performed.Serum bone metabolism indexes BALP,PINP,CTX-1 and TRAP levels were detected by ELISA.Results During the experiment,there were no significant changes in body weight and main organs,no significant differences in organ index(P>0.05),no significant abnormalities in heart,liver,spleen,lung and kidney organs wer observed.The bone mineral density(BMD)of femur and vertebrae in the ovinus placenta powder intervention group was significantly higher than that in the Ctrl group.Micro-CT reconstruction result showed that compared with Ctrl group,bone volume fraction,total bone surface volume,trabecular number and trabecular thickness in group M were significantly increased(P<0.05),and extremely significantly increased in group H(P<0.01).Trabecular separation in group M was significantly decreased(P<0.05),and extremely significantly decreased in group H(P<0.01).Pathological observation of femur showed that the number of bone trabeculae increased in different degrees in each intervention group,and the effect of H group was the most obvious.The result of bone biomechanical test showed that the maximum load and compressive strength of femur in groups M and H could be significantly increased.The result of double fluorescence labeling showed that compared with the Ctrl group,the fluorescence labeling spacing of each drug administration group was increased,and the M and H groups were more obvious.The detection result of serum bone metabolism indexes showed that compared with Ctrl group,the levels of bone formation indexes PINP and BALP in groups M and H were significantly increase

关 键 词：羊胎盘粉 峰值骨量 骨形成 骨代谢 骨强度 

分 类 号：R285.5[医药卫生—中药学]