重复经颅磁刺激对小鼠海马谷氨酸能神经元和γ-氨基丁酸能神经元兴奋性影响的研究  

Effect of repeated transcranial magnetic stimulation on excitability of glutaminergic neurons and gamma-aminobutyric neurons in mouse hippocampus

作　　者：王佳乐 丁冲 付蕊 张泽 赵峻峤 朱海军 WANG Jiale;DING Chong;FU Rui;ZHANG Ze;ZHAO Junqiao;ZHU Haijun(School of Health Sciences&Biomedical Engineering,Hebei University of Technology,Tianjin 300130,P.R.China;Hebei Key Laboratory of Bioelectromagnetics and Neural Engineering,Hebei University of Technology,Tianjin 300130,P.R.China;Key Laboralory of Digital Medical Engincering of Hebei Province,School of Electronic Information Engineering,Hebei Universily,Baoding,Hebei 071002,P.R.China)

机构地区：[1]河北工业大学生命科学与健康工程学院,天津300130 [2]河北工业大学河北省生物电磁学与神经工程重点实验室,天津300130 [3]河北大学电子信息工程学院河北省数字医疗工程重点实验室,河北保定071002

出　　处：《生物医学工程学杂志》2025年第1期73-81,共9页Journal of Biomedical Engineering

基　　金：国家自然科学基金(52077057,52207251);河北省自然科学基金(F2022202023)。

摘　　要：重复经颅磁刺激(rTMS)是目前常用的脑刺激技术之一。为探究rTMS对不同类型神经元兴奋性的影响,本研究从电生理角度开展rTMS对小鼠认知功能和海马谷氨酸能神经元、γ-氨基丁酸能神经元兴奋性影响的研究。本研究将小鼠随机分为谷氨酸能对照组、谷氨酸能磁刺激组、γ-氨基丁酸能对照组、γ-氨基丁酸能磁刺激组,对四组小鼠注射腺相关病毒标记两类神经元并植入光纤。刺激组接受14天刺激,对照组接受14天伪刺激。光纤记录小鼠钙离子荧光强度变化。行为学实验探究小鼠认知功能变化。脑片膜片钳系统检测神经元动作电位特性变化。结果发现rTMS可显著提高小鼠的认知功能,提高海马脑区谷氨酸能神经元、γ-氨基丁酸能神经元集群的钙离子荧光变化幅度,以及增强谷氨酸能神经元、γ-氨基丁酸能神经元动作电位相关指标。结果提示,rTMS可通过提高海马谷氨酸能神经元和γ-氨基丁酸能神经元的兴奋性来提高小鼠的认知能力。Repeated transcranial magnetic stimulation(rTMS)is one of the commonly used brain stimulation techniques.In order to investigate the effects of rTMS on the excitability of different types of neurons,this study is conducted to investigate the effects of rTMS on the cognitive function of mice and the excitability of hippocampal glutaminergic neurons and gamma-aminobutyric neurons from the perspective of electrophysiology.In this study,mice were randomly divided into glutaminergic control group,glutaminergic magnetic stimulation group,gammaaminobutyric acid energy control group,and gamma-aminobutyric acid magnetic stimulation group.The four groups of mice were injected with adeno-associated virus to label two types of neurons and were implanted optical fiber.The stimulation groups received 14 days of stimulation and the control groups received 14 days of pseudo-stimulation.The fluorescence intensity of calcium ions in mice was recorded by optical fiber system.Behavioral experiments were conducted to explore the changes of cognitive function in mice.The patch-clamp system was used to detect the changes of neuronal action potential characteristics.The results showed that rTMS significantly improved the cognitive function of mice,increased the amplitude of calcium fluorescence of glutamergic neurons and gamma-aminobutyric neurons in the hippocampus,and enhanced the action potential related indexes of glutamergic neurons and gamma-aminobutyric neurons.The results suggest that rTMS can improve the cognitive ability of mice by enhancing the excitability of hippocampal glutaminergic neurons and gamma-aminobutyric neurons.

关 键 词：重复经颅磁刺激 谷氨酸能神经元 Γ-氨基丁酸能神经元 学习记忆 在体光纤记录 脑片膜片钳 

分 类 号：R338[医药卫生—人体生理学]