‘突尼斯软籽’石榴叶片愈伤组织诱导条件研究  

Study on Callus Induction Conditions of Pomegranate Leaves of‘Tunisia Soft Seed’

作　　者：王虹 贾毛毛 陶爱丽[2] 李玉英[2] 源朝政 杨玲 高小峰 WANG Hong;JIA Maomao;TAO Aili;LI Yuying;YUAN Chaozheng;YANG Ling;GAO Xiaofeng(Nanyang Academy of Sciences,Nanyang Key Laboratory of Pomegranate Breeding,Nanyang 473000,China;Nanyang Normal University,Henan Soft Seed Pomegranate Engineering Research Center,Nanyang 473000,China)

机构地区：[1]南阳市科学院,南阳市石榴新品种选育重点实验室,河南南阳473000 [2]南阳师范学院,河南省软籽石榴工程研究中心,河南南阳473000

出　　处：《中国果菜》2025年第2期51-55,共5页China Fruit & Vegetable

基　　金：河南省科技厅高等学校学科创新引智基金(CXJD2019001);河南省科技创新体系建设项目(192207310029);河南省科技攻关项目(232102110009);河南省科技攻关项目(232102110224)。

摘　　要：为了开展石榴遗传转化研究,以‘突尼斯软籽’石榴为试材,叶片为外植体,采用响应面回归分析方法研究了不同生长调节物质对离体叶片愈伤组织再生的影响。结果表明,‘突尼斯软籽’石榴叶片愈伤组织诱导的最佳培养基为MS+6-BA 1.5 mg/L+2,4-D 0.5 mg/L+NAA 0.3 mg/L,诱导率可达83.9%,且愈伤组织生长状况良好。本研究建立了‘突尼斯软籽’石榴高效的离体快繁体系,为石榴繁育技术的改善提供了理论依据。In order to develop genetic transformation test of pomegranate,in this paper,using leaves in‘Tunisia soft-seed’pomegranate as explants,the different growth regulators on callus regeneration of leaves by response surface method was studied.The results showed that the optimal medium was MS+1.0 mg/L 6-BA+1.0 mg/L 2,4-D+0.2 mg/L NAA,the induction rate was 83.9%,and most of the callus grew well.At the same time,a high efficient rapid propagation system of‘Tunisia soft-seed’pomegranate was established.And it also provided a theoretical basis for improving pomegranate breeding technology.

关 键 词：‘突尼斯软籽’石榴 植物生长调节剂 愈伤组织 响应面法 

分 类 号：S665.4[农业科学—果树学]