TPU/HM-531改性材料制备及生物相容性评价  

Preparation and Biocompatibility Evaluation of TPU/HM-531 Blends

作　　者：林泽铨 王庆琳 陈晓邦 欧阳阅翰 田慧 赵晨 利希婷 余子卉 朱锐钿 李荣荣 阳范文[1] LIN Ze-quan;WANG Qin-ling;CHEN Xiao-bang;OUYANG Yue-han;TIAN Hui;ZHAO Chen;LI Xi-ting;YU Zi-hui;ZHU Rui-tian;LI Rong-rong;YANG Fan-wen(School of Biomedical Engineering,Guangzhou Medical University,Guangzhou 511436,Guangdong,China;The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510799,Guangdong,China;Guangzhou Inspection Testing and Certification Group Co.,Ltd.,Guangzhou 511447,Guangdong,China)

机构地区：[1]广州医科大学生物医学工程学院,广东广州511436 [2]广州医科大学附属第五医院,广东广州510799 [3]广州检验检测认证集团有限公司,广东广州511447

出　　处：《合成材料老化与应用》2024年第6期20-23,共4页Synthetic Materials Aging and Application

基　　金：广东省药监局科技项目(2023YDZ12);广东省普通高校质量工程项目(2023年度医疗器械现代产业学院);广州医科大学学生创新能力提升计划项目(2023-50);广州医科大学2023年度质量工程项目(2023ZLGC129)。

摘　　要：将HM-531亲水改性剂与TPU进行熔融共混,制备TPU/HM-531亲水改性材料,对其亲水性能和细胞毒性进行研究。结果表明:随着HM-531用量增加,材料的接触角逐渐降低;当其用量为2.5%和7.5%时,改性材料的接触角分别为37.89°和26.59°,亲水改性效果良好。HM-531用量为2.5%~7.5%的改性材料组,1d、3d和5d的细胞相对增长率≥90%,显示无细胞毒性。实验材料组的浸提液稀释50倍时,显微镜下观察到大部分细胞呈现梭型、生长状态良好;活细胞/死细胞荧光检测发现大部分细胞显示绿色,仅有少数几个细胞呈红色,与阴性对照组无显著性差异。在TPU中添加2.5%~7.5%的HM-531,可制备亲水性能优异、生物相容性良好的改性材料。TPU/HM-531 hydrophilic modified blends were prepared by melt blending with HM-531 as a hydrophilic modifier for TPU.The hydrophilicity and cytotoxicity of the blends were investigated.As the content of HM-531 content increases,the contact angle of the blends gradually decreases.They are 37.89°and 26.59°respectively for blend with 2.5% and 7.5% content of HM-531,indicating a good hydrophilic modification effect.The blends with HM-531 content ranging from 2.5%to 7.5% showed no cytotoxicity with a relative cell growth rate of≥90% after 1 day,3 days,and 5 days of cultivation.When the extraction solution of the experimental blend group was diluted 50 times,most of the cells were observed to be spindle shaped and in good growth state under the microscope.The fluorescence detection of live/dead cells showed that the majority of cells showed green color and only a few cells showed red color,which shows no significant difference compared to the negative control.Blends with excellent hydrophilicity and good biocompatibility can be prepared by adding 2.5%~7.5%HM-531 to TPU.

关 键 词：热塑性聚氨酯(TPU) 聚氧化乙烯-氧化丙烯共聚物 亲水改性 接触角 生物相容性 

分 类 号：TQ322[化学工程—合成树脂塑料工业] TB324[一般工业技术—材料科学与工程]