一株高效溶磷菌株的筛选及对亚麻促生效应的研究  

Screening of a High Efficiency Phosphorus Dissoluble Strain and Its Growth-Promoting Effect to Flax

作　　者：胡云龙[1,2,3] 陈文晶 徐伟慧 王志刚[1,2,3] HU Yun-long;CHEN Wen-jing;XU Wei-hui;WANG Zhi-gang(Schl.of Life Sci.&Agric.&Forest.,Qiqihar Uni.,Qiqihar 161006;Heilongjiang Prov.Agric.Microb.Prep.Industrializ'n Tech.Innov.Ctr.,Qiqihar 161006;Engin.Res.Ctr.of Hemp&Hemp Prod.in Cold Reg.,Minist.of Educ.,Qiqihar 161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]黑龙江省农业微生物制剂产业化技术创新中心,黑龙江齐齐哈尔161006 [3]寒区麻及制品教育部工程研究中心,黑龙江齐齐哈尔161006

出　　处：《微生物学杂志》2025年第1期59-67,共9页Journal of Microbiology

基　　金：黑龙江省省属高等学校基本科研业务费(145309336);大学生创新创业项目(202010232148)。

摘　　要：溶磷菌能够显著提升土壤中可溶性磷含量,促进作物生长,增加肥力。从工业大麻根际土壤中进行溶磷菌筛选,通过测定溶磷量、发芽率、发芽势筛选出一株高效溶磷菌并对其进行鉴定,同时对其吲哚乙酸(indole acetic acid,IAA)分泌量以及对亚麻生长的影响进行测定。结果显示,筛选得到的JX1菌株经鉴定为Klebsiella sp.,其溶磷能力较强且具有功能多样性,最大溶磷量为181.70 mg/L,能显著提高亚麻种子发芽势与亚麻幼苗总根长,具有IAA分泌能力,其最大分泌量为49.94 mg/L,亚麻经JX1菌株浇灌后,能显著提升其株高、地下部干鲜重、总根长、根表面积、根体积与直径<0.5 mm细根所占的比例,显著降低根系平均直径,对亚麻地下部具有较好促生效果。本研究通过筛选得到的菌株JX1(Klebsiella sp.)具有较好的溶磷以及分泌IAA的能力,能够有效促进亚麻根系生长,优化根系的形态与组成,提高亚麻根系对水分和营养的吸收利用,丰富了亚麻专用微生物肥料的高效菌株资源。Phosphorus dissolvable bacteria can significantly increase the soluble phosphorus content in soil,promote crop growth and increase fertility.In this study,phosphorus dissolvable bacteria were screened from the hemp rhizosphere soil,and a high efficiency phosphorus dissolvable bacterium was screened and identified by methods such as phosphorus soluble amount,germination rate,and germination potential,indole acetic acid(IAA)secretion and the effect of flax growth were measured.The results showed that the screened JX1 strain was identified as Klebsiella sp.,with strong phosphorus dissolubility and functional diversity.The maximum phosphorus dissolvable amount was 181.70 mg/L,which could significantly improve the germination potential of flax seeds and the total root length of flax seedlings,having the ability to secrete IAA,the maximum IAA secretion at 49.94 mg/L,45 days after irrigation of JX1 strain,it could significantly increase the flax plant height,underground dry and fresh weight,total root length,root surface area,root volume and the proportion of fine roots with diameter<0.5 mm,significantly reduce the average root diameter,which had a better effect on promoting the growth of the underground part of flax.This study showed that strain JX1(Klebsiella sp.)has better ability to dissolve phosphorus and secrete IAA,which can effectively promote the growth of flax roots,optimize the morphology and composition of roots,and improve the absorption and utilization of water and nutrients by flax roots.Provide high efficiency strain resources for the research and development of microbial fertilizers for flax.

关 键 词：溶磷 亚麻 根系 吲哚乙酸(IAA) 促生效应 

分 类 号：Q93-31[生物学—微生物学]