我国主要芒果产区间座壳属真菌的分离鉴定及致病性研究  

Identification and Pathogenicity of Diaporthe Fungal Species in Major Mango Producing Areas in China

作　　者：王睿 张玉杰 孙文秀[2] 唐利华[1] 黄穗萍[1] 郭堂勋[1] 陈小林[1] 李其利[1] WANG Rui;ZHANG Yu-jie;SUN Wen-xiu;TANG Li-hua;HUANG Sui-ping;GUO Tang-xun;CHEN Xiao-lin;LI Qi-li(Inst.of Plant Protect.,Guangxi Zhuang Auto.Reg.Acad.of Agric.Sci./Key Lab.of Green Prevent.&Ctrl.on Fruits&Veget.in S.China Minist.of Agric.&Rural Aff./Guangxi Key Lab.of Biol.for Crop Dis.&Insect Pests,Nanning 530007;Coll.of Life Sci.,Yangtze Uni.,Jingzhou 434025)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁530007 [2]长江大学生命科学学院,湖北荆州434025

出　　处：《微生物学杂志》2025年第1期76-84,共9页Journal of Microbiology

基　　金：财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-31)。

摘　　要：间座壳属真菌是芒果叶斑病的主要病原菌之一,严重影响芒果的产量和品质。为明确引起我国芒果叶斑病的病原菌种类及其优势种群,本研究于2018年从广东、广西、云南、海南和福建五个芒果主产区采集病叶,通过组织分离纯化,共分离到43个间座壳属真菌,结合形态学和多基因(ITS、HIS、TEF 1-α和TUB2)系统发育分析对其中28个代表性菌株进行鉴定,并采用离体叶片接种法进行致病性测定。结果表明,28株代表菌株被鉴定为5个菌种:Diaporthe ueckerae、D.endophytica、D.tulliensis、D.yunnanensis和D.arecae,其中D.arecae是优势种,占比64%。致病性测定结果显示这些间座壳属菌株对芒果叶片均具有致病性,且致病力存在显著差异。研究结果为深入研究间座壳属真菌引起的芒果叶斑病的发生规律和防治技术提供参考。Fungal strains of Diaporthe spp.are ones of the main pathogens of mango leaf spot,which seriously affects the yield and quality of fruits.In order to determine the pathogens and dominant populations that causes mango leaf spot in China,samples were collected from five major mango producing regions in Guangdong,Guangxi,Yunnan,Hainan and Fujian in this study in 2018.Forty-three fungal strains of Diaporthe spp.were isolated from diseased tissues,and twenty-eight representative strains were identified through morphological characteristics and multigene(ITS,HIS,TEF 1-α and TUB2)phylogenetic analysis.Pathogenicity assays were also conducted on detached mango leaves.Twenty-eight purified fungal isolates were obtained and then identified as Diaporthe ueckerae,D.endophytica,D.tulliensis,D.yunnanensis and D.arecae,respectively.D.arecae were the dominant species,accounting for 64%.Pathogenicity test confirmed that these strains of Diaporthe spp.were pathogenic to mango leaves with significant differences in pathogenicity.The results provided scientific basis for further study on the occurrence regularity and control technology of mango leaf spot caused by Diaporthe spp.

关 键 词：芒果 叶斑病 间座壳属 真菌鉴定 致病力 

分 类 号：Q93-331[生物学—微生物学]