结核分枝杆菌RpfB及RpfE蛋白的生物信息学分析  

Bioinformatics Analysis of RpfB and RpfE Proteins of Mycobacterium tuberculosis

作　　者：杨雨婷 杨国平 王艳 龚丽坤 YANG Yu-ting;YANG Guo-ping;WANG Yan;GONG Li-kun(Clin.Lab.of Trad.Chinese Med.Hosp.of Chuxiong,Chuxiong 675000;Chuxiong Yi Nationality Med.Res.Inst.,Chuxiong 675000;Teach.&Res.Div.of Med.Microbiol.&Immunol.,Schl.of Basic Med.,Dali Uni.,Dali 671000)

机构地区：[1]楚雄州中医医院检验科,云南楚雄675000 [2]楚雄州彝族医药研究院,云南楚雄675000 [3]大理大学基础医学院医学微生物学及免疫学教研室,云南大理671000

出　　处：《微生物学杂志》2025年第1期106-116,共11页Journal of Microbiology

基　　金：云南省地方本科高校联合专项面上项目(2017FH001-085)。

摘　　要：运用生物信息学方法预测RpfB和RpfE蛋白的生物学特性及抗原表位,并分析其作为新型结核疫苗候选抗原的潜力。从NCBI数据库中获取Rv1009、Rv2450c基因的碱基序列,从Uniprot数据库中获取Rv1009基因编码蛋白RpfB及Rv2450c基因编码蛋白RpfE的氨基酸序列,运用生物信息学分析软件ORFfinder、ProtParam、ProtScale、TMPRED、TMHMM、SignalP 5.0、NetNGlyc 1.0、YinOYang 1.2、NetPhos 3.1、STRING、SOPMA、SWISS-MODEL、DNAStar、SYFPEITHI、NetCTL 1.2及Net MHCII pan 4.0对RpfB及RpfE蛋白的理化性质、亲疏水性、结构、信号肽、糖基化/磷酸化位点、跨膜结构、互作蛋白及抗原表位进行分析。结果表明,Rv1009基因全长1089 bp,有5个开放阅读框,其编码蛋白RpfB由362个氨基酸组成,为含有信号肽的疏水性跨膜蛋白,其二级结构以无规则卷曲为主,该蛋白含有1个N-糖基化位点、29个磷酸化位点、8个优势CTL细胞抗原表位、14个优势Th细胞抗原表位及10个优势B细胞抗原表位;Rv2450c基因全长519 bp,有1个开放阅读框,其编码蛋白RpfE由172个氨基酸组成,为含有信号肽的亲水性蛋白,无跨膜结构,其二级结构以无规则卷曲为主,该蛋白含有2个N-糖基化位点、9个磷酸化位点、4个优势CTL细胞抗原表位、6个优势Th细胞抗原表位及7个优势B细胞抗原表位。RpfB及RpfE蛋白具有较好的免疫原性及多个优势T、B细胞抗原表位,可作为Mtb多肽疫苗研制的候选优势蛋白。Using bioinformatics methods to predict the biological characteristics and epitopes of RpfB and RpfE protein,and to analyze their potential as candidate antigens for novel tuberculosis vaccines.Base sequences of Rv 1009 and Rv 2450 c genes were obtained from the NCBI database and amino acid sequences of Rv 1009 gene coding protein RpfB and Rv 2450 c gene coding protein RpfE were obtained from the Uniprot database.Bioinformatics analysis software,ORFfinder,ProtParam,ProtScale,TMPRED,TMHMM,SignalP 5.0,NetNGlyc 1.0,YinOYang 1.2,NetPhos 3.1,STRING,SOPMA,SWISS-MODEL,DNAStar,SYFPEITHI,NetCTL 1.2 and Net MHCII pan 4.0,were used to analysis physio-chemical properties,hydrophilicity,structure,signal peptide,glycosylation and phosphorylation sites,transmembrane structure,epitopes and protein interaction of RpfB and RpfE protein.Gene Rv 1009 at full length 1089 bp with 5 open reading frames,encoded protein RpfB consisting of 362 amino acids.It was a hydrophobic transmembrane protein containing a signal peptide,and its secondary structure is mainly irregular curl.The protein contains 1 N-glycosylation site,29 phosphorylation sites,8 dominant CTL cell epitopes,14 dominant Th cell epitopes as well as 10 dominant B cell epitopes.Gene Rv 2450 c at full length 519 bp with 1 open reading frames,encoded protein RpfE consisting of 172 amino acids.It was a hydrophilic protein containing a signal peptide,and its secondary structure is mainly irregular curl.The protein contains 2 N-glycosylation site,9 phosphorylation sites,4 dominant CTL cell epitopes,6 dominant Th cell epitopes and 7 dominant B cell epitopes.RpfB and RpfE proteins have good immunogenicity and several dominant T and B cell epitopes,which can be used as candidate dominant proteins in the development of Mtb polypeptide vaccine.

关 键 词：结核分枝杆菌(Mycobacterium tuberculosis Mtb) RpfB RpfE 生信分析 抗原表位 多肽疫苗 

分 类 号：Q936[生物学—微生物学]