慢性乙型肝炎患者NK细胞趋化因子受体表达及其与病毒载量、肝硬化的关系分析  

Analysis of NK cell chemokine receptor expression and its relationship with viral load and cirrhosis in patients with chronic hepatitis B

作　　者：郝邯冬 冯晓丽[1] Hao Handong;Feng Xiaoli(Department of Clinical Laboratory,Anyang Hospital of Traditional Chinese Medicine,Anyang,Henan 455000,China)

机构地区：[1]安阳市中医院检验科,河南安阳455000

出　　处：《齐齐哈尔医学院学报》2025年第3期249-253,共5页Journal of Qiqihar Medical University

摘　　要：目的 分析慢性乙型肝炎(CHB)患者自然杀伤细胞(NK)趋化因子受体表达及其与病毒载量、肝硬化的关系。方法 选择2019年1月—2020年1月本院收治的150例CHB患者作为研究对象,用流式细胞术分析2组外周血NK细胞表面趋化因子受体1(CCR1)、CXC趋化因子受体6(CXCR6)表达水平,实时荧光定量聚合酶链反应(qPCR)检测CHB患者血清HBV-DNA水平,并对其CCR1、CXCR6表达水平与HBV-DNA的相关性作Pearson分析;随访3年,通过肝穿刺诊断CHB组患者是否合并肝硬化,并以此为依据分为肝硬化组和无肝硬化组,并比较2组患者的CCR1、CXCR6表达水平差异,COX回归分析探讨CHB患者肝硬化的影响因素。结果 CCR1与HBV-DNA水平呈负相关(P<0.001),CXCR6与HBV-DNA水平呈正相关(P<0.001)。与无肝硬化组比较,肝硬化组CHB病程、饮酒史比例、HBV DNA、CXCR6表达水平较高,且CCR1表达水平较低(P<0.05);CHB病程、饮酒史、HBV DNA、CXCR6与HBV-DNA水平是CHB发生肝硬化的影响因素(P<0.05)。结论 CHB患者NK细胞趋化因子受体CCR1、CXCR6表达水平及其与病毒载量相关,且是肝硬化发生的影响因素。Objective To analyze the expression of natural killer cell(NK)chemokine receptors in patients with chronic hepatitis B(CHB)and their relationship with viral load and cirrhosis.Methods A total of 150 CHB patients admitted to our hospital from January 2019 to January 2020 were selected as subjects.Flow cytometry was used to analyze the expression levels of surface chemokine receptor 1(CCR1)and CXC chemokine receptor 6(CXCR6)on peripheral blood NK cells.Real-time fluorescence quantitative PCR(qPCR)was employed to detect serum HBV-DNA levels in CHB patients,and Pearson analysis was conducted to investigate the correlation between CCR1,CXCR6 expression levels,and HBV-DNA.Patients were followed up for 3 years,and liver biopsy was used to diagnose the presence of cirrhosis in CHB patients.The expression levels of CCR1 and CXCR6 were compared between the cirrhosis and non-cirrhosis groups,and COX regression analysis was used to explore the influencing factors of cirrhosis in CHB patients.Results CCR1 was negatively correlated with HBV-DNA levels(P<0.001),while CXCR6 was positively correlated with HBV-DNA levels(P<0.001).Compared to the non-cirrhotic group,the cirrhotic group had a longer duration of CHB,a higher proportion of alcohol consumption history,higher expression levels of HBV-DNA and CXCR6,and lower expression levels of CCR1(P<0.05).The duration of CHB,history of alcohol consumption,HBV DNA,and CXCR6 were identified as influencing factors for the occurrence of cirrhosis in CHB patients(P<0.05).Conclusions The expression levels of NK cell chemokine receptors CCR1 and CXCR6 in CHB patients,and their correlation with viral load,are factors influencing the occurrence of cirrhosis.

关 键 词：慢性乙型肝炎 自然杀伤细胞 趋化因子 病毒载量 肝硬化 相关性 

分 类 号：R512.62[医药卫生—内科学]