Bta-miR-134靶基因预测及生物信息学分析  

Target gene prediction and bioinformatics analysis of bta-miR-134

作　　者：许浩天 赵雅菲 于月通 李静 齐萌[1] XU Haotian;ZHAO Yafei;YU Yuetong;LI Jing;QI Meng(College of Animal Science and Technology,Tarim University,Alar,Xinjiang 843300)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300

出　　处：《塔里木大学学报》2025年第1期29-37,共9页Journal of Tarim University

基　　金：塔里木大学校长基金硕士人才项目(TDZKSS202219)。

摘　　要：本研究对LPS与钙离子共处理的奶牛乳腺上皮细胞RNA样本以及LPS处理的奶牛乳腺上皮细胞RNA样本进行miRNA测序,发现16个差异表达miRNA,其中表达水平上调miRNA 1个,表达水平下调miRNA 15个。上调miRNA为bta-miR-134,其可能在钙离子抑制奶牛乳腺上皮细胞炎症反应进程中发挥关键作用。因此,对奶牛乳腺上皮细胞来源bta-miR-134的靶基因进行预测并开展生物信息学分析,为bta-miR-134的功能验证奠定研究基础。使用miRBase数据库分析bta-miR-134的成熟序列保守性;使用Targetscan数据库预测bta-miR-134的靶基因;使用R语言中clusterProfiler包对bta-miR-134靶基因进行GO和KEGG富集分析;使用STRING数据库分析bta-miR-134靶基因的蛋白互作关系。Bta-miR-134的成熟序列在不同物种间高度保守;预测到bta-miR-134的靶基因共计171个;GO功能富集结果显示,靶基因主要富集蛋白丝氨酸/苏氨酸激酶活性、蛋白激酶活性、蛋白丝氨酸激酶活性等分子功能;KEGG通路富集结果显示,靶基因主要参与Ras信号通路、神经营养因子信号通路、人类免疫缺陷病毒1型感染和恰加斯病等相关信号通路;蛋白互作分析表明KRAS、PIK3CA、CASP8这3个基因与其他基因的连通度最高。Bta-miR-134可能靶向KRAS、PIK3CA、CASP8等关键基因,由Ras信号通路、MAPK信号通路和Toll样受体信号通路进一步介导,从而调控奶牛乳腺上皮细胞炎症反应。本研究为进一步揭示奶牛乳腺上皮细胞中钙离子、miRNA与炎症信号通路之间的调控机制提供重要数据。In this study,the RNA samples of mammary epithelial cells of cows co-treated with LPS and calcium ions as well as the RNA samples of mammary epithelial cells of cows treated with LPS were subjected to miRNA sequencing.Sixteen differentially expressed miRNAs were identified,of which one exhibited increased expression level and fifteen displayed decreased expression.The miRNA that up-regulated was bta-miR-134,which may play a pivotal role in the inhibition of the inflammatory response process of cow mammary epithelial cells by calcium ions.Accordingly,the target genes of bta-miR-134 derived from cow mammary epithelial cells were predicted,and bioinformatics analysis was conducted to establish a research foundation for the functional verification ofbta-miR-134. The miRBase database was employed for the analysis of the maturation sequence conservation of bta-miR-134. In orderto predict the target genes of bta-miR-134,the Targetscan database was utilised. The clusterProfiler package in R was employedto conduct GO and KEGG enrichment analyses of the target genes of bta-miR-134. The STRING database was employed for theanalysis of protein interactions among the target genes of bta-miR-134. The mature sequence of bta-miR-134 exhibits high conservation among different species. A total of 171 target genes of bta-miR-134 were predicted. The enrichment results of GO demonstratedthat the target genes were predominantly enriched in protein serine/threonine kinase activity,protein kinase activity,protein serinekinase activity and other molecular functions. The KEGG pathway enrichment results indicated that the target genes were predominantly associated with the Ras signaling pathway,neurotrophic factor signaling pathway,human immunodeficiency virus type 1 infection and Chagas disease. The protein interaction analysis revealed that KRAS,PIK3CA and CASP8 exhibited the highest degree ofconnectivity with other genes. Bta-miR-134 may target key genes,such as KRAS,PIK3CA and CASP8,and is further mediated bythe Ras signaling pathway,th

关 键 词：bta-miR-134 靶基因 生物信息学分析 

分 类 号：S852.23[农业科学—基础兽医学]