格氏乳球菌SHAMU-LG6对葡萄球菌的拮抗活性研究  

Study on the antagonistic activity of Lactococcus garvieae SHAMU-LG6 against Staphylococcus

作　　者：翁胜男 冷贵云 刘颖 王亚武 李昕[1] 姚杰[1] 周强[1] 唐伟 Weng Shengnan;Leng Guiyun;Liu Ying;Wang Yawu;Li Xin;Yao Jie;Zhou Qiang;Tang Wei(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601;High Magnetic Field Laboratory,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031)

机构地区：[1]安徽医科大学第二附属医院检验科,合肥230601 [2]中国科学院合肥物质科学研究院强磁场科学中心,合肥230031

出　　处：《安徽医科大学学报》2025年第2期195-200,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82102460);安徽医科大学研究生科研与实践创新项目(编号:YJS20230190);安徽医科大学第二附属医院临床研究培育计划项目(编号:2021LCZD13);安徽医科大学校科研基金项目(编号:2022xkj197)。

摘　　要：目的探索格氏乳球菌SHAMU-LG6对葡萄球菌的拮抗活性。方法利用VITEK 2 GP鉴定卡、Microflex LT MALDI-TOF质谱分析仪和16S rDNA扩增测序3种方法共同鉴定菌株种属。牛津杯法抑菌试验检测格氏乳球菌SHAMU-LG6对不同葡萄球菌的拮抗活性;XAD16非离子型大孔树脂吸附、梯度乙醇洗脱和旋转蒸发干燥初步分离纯化抗菌活性成分。结果格氏乳球菌SHAMU-LG6对耐甲氧西林金黄色葡萄球菌、甲氧西林敏感金黄色葡萄球菌、表皮葡萄球菌、腐生葡萄球菌、路邓葡萄球菌、人葡萄球菌、头状葡萄球菌、沃氏葡萄球菌等均具有拮抗效应,抑菌指数分别为3.3、3.0、4.3、2.0、4.0、3.5、3.8和3.5。格氏乳球菌SHAMU-LG6分泌的抗菌活性成分主要存在于70%和80%乙醇洗脱物中。结论格氏乳球菌SHAMU-LG6对葡萄球菌具有强拮抗效应,其分泌的抗菌活性成分有望成为新型抗菌药物开发的先导化合物。Objective To investigate the antagonistic activity of Lactococcus garvieae SHAMU-LG6 against Staphylococcus.Methods VITEK 2 GP identification card,Microflex LT MALDI-TOF mass spectrometer and 16S rDNA amplification sequencing were used to identify the strain species.The antagonistic activity of L.garvieae SHAMU-LG6 against different Staphylococcus was detected by Oxford cup method for bacterial inhibition;the antimicrobial active components were preliminarily isolated and purified by adsorption on XAD16 nonionic macroporous resin,gradient ethanol elution and rotary evaporation drying.Results L.garvieae SHAMU-LG6 exhibited potent antagonistic effect against methicillin-resistant Staphylococcus aureus,methicillin-susceptible S.aureus,S.epidermidis,S.saprophyticus,S.lugdunensis,S.hominis,S.capitis and S.warneri,with inhibitory indices of 3.3,3.0,4.3,2.0,4.0,3.5,3.8,and 3.5,respectively.The antimicrobial active components produced by L.garvieae SHAMU-LG6 were mainly present in 70%and 80%ethanol eluates.Conclusion L.garvieae SHAMU-LG6 exhibits a potent antagonistic effect on Staphylococcus,and the antimicrobial active components produced by it are expected to be a lead compound for the development of novel antimicrobial agents.

关 键 词：格氏乳球菌 葡萄球菌 拮抗活性 抗菌活性成分 分离纯化 16S rDNA 牛津杯法 

分 类 号：Q939.92[生物学—微生物学] R378.11[医药卫生—病原生物学]