延胡索总生物碱的提取及镇痛抗炎作用研究  

Extraction and Analgesic and Anti-inflammatory Effects of the Total Alkaloids of Corydalis yanhusuo

作　　者：陈思懿 谷雨薇 魏永孝 王秦莉 代敏 蒲忠慧 CHEN Siyi;GU Yuwei;WEI Yongxiao;WANG Qinli;DAI Min;PU Zhonghui(School of Laboratory Medicine,Chengdu Medical College,Chengdu 610500,China;Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin Food,Chengdu 610500,China)

机构地区：[1]成都医学院检验医学院,成都610500 [2]四川省动物源性食品兽药残留防控技术工程实验室,成都610500

出　　处：《世界中医药》2024年第22期3431-3436,共6页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82204601);四川省中医药管理局面上项目(2023MS084;20232D02);“成医英才”登峰计划人才项目(2024qnGzn14)。

摘　　要：目的:优选延胡索总生物碱的提取工艺,并考察其镇痛抗炎活性。方法:在单因素实验基础上,以乙醇浓度、液料比、提取温度、提取时间为影响因素,用高效液相色谱法(HPLC)测定的脱氢紫堇碱含量为指标,正交试验L 9(34)优化提取工艺。采用醋酸扭体法和热板法评价延胡索总生物碱的镇痛活性,采用二甲苯致小鼠耳肿胀法和鸡蛋清致小鼠足肿胀法考察其抗炎活性。结果:延胡索总生物碱最佳提取工艺:以1∶25的料液比,80%浓度乙醇在50℃下提取3.5 h。延胡索总生物碱能明显降低冰醋酸诱发的小鼠扭体次数,提高小鼠的痛阈值,抑制二甲苯所致的小鼠耳肿胀和鸡蛋清所致的小鼠足肿胀(P<0.01或P<0.05)。结论:采用乙醇加热回流提取延胡索总生物碱、以HPLC测定的脱氢紫堇碱含量为指标优化的提取工艺简便可行、稳定可靠,且延胡索总生物碱具有显著的体内镇痛抗炎活性。Objective:To optimize the extraction process of total alkaloids from Corydalis yanhusuo and evaluate its analgesic and anti-inflammatory activities.Methods:Based on single-factor experiments,the ethanol concentration,liquid-to-material ratio,extraction temperature,and extraction time were selected as influencing factors.The dehydrocorydaline content,measured by high-performance liquid chromatography(HPLC),was used as the index to optimize the extraction process via an orthogonal L 9(34)experiment.The analgesic activity of total alkaloids from C.yanhusuo was evaluated using the acetic acid writhing test and the hot plate method.The anti-inflammatory activity was assessed using the xylene-induced ear swelling test and the egg albumin-induced paw swelling test in mice.Results:The optimal extraction process for total alkaloids from C.yanhusuo was a 1∶25 material-to-solvent ratio,with 80%ethanol,extracted at 50℃for 3.5 h.The total alkaloids significantly reduced the number of writhing movements induced by acetic acid,increased the pain threshold in mice,and inhibited the ear swelling caused by xylene and paw swelling induced by egg albumin(P<0.01 or P<0.05).Conclusion:The ethanol reflux extraction method,optimized based on the dehydrocorydaline content measured by HPLC,is simple,feasible,stable,and reliable.Moreover,the total alkaloids from C.yanhusuo exhibit significant in vivo analgesic and anti-inflammatory activities.

关 键 词：延胡索 总生物碱 提取工艺 镇痛 抗炎 高效液相色谱法 正交试验 工艺优化 

分 类 号：R282[医药卫生—中药学]