血尿安片对IgA肾病大鼠肾小球系膜细胞增生的影响  

Effect of Xueniaoan Tablets on the Proliferation of Glomerular Messangial Cells in Rats with IgA Nephropathy

作　　者：佟彤 叶波 郎睿[3] 徐建龙[3] 王洪霞[3] TONG Tong;YE Bo;LANG Rui;XU Jianlong;WANG Hongxia(Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China;Beijing Haidian Balizhuang Community Health Service Center,Beijing 100085,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100191,China)

机构地区：[1]中国中医科学院眼科医院,北京100040 [2]北京市海淀区八里庄卫生服务中心,北京100085 [3]中国中医科学院西苑医院,北京100191

出　　处：《世界中医药》2024年第22期3473-3478,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(81873300)——基于PI3K/AKT/mTOR及AMPK/mTOR/ULK1信号通路调控自噬的交互作用探讨加味黄茂赤风汤对足细胞损伤的保护机制。

摘　　要：目的:观察血尿安片对IgA肾病大鼠的治疗作用及对肾小球系膜细胞增生的影响。方法:30只斯泼累格·多雷(SD)大鼠数字表法随机分为对照组、模型组、中药组,建立免疫球蛋白A肾病(IgAN)模型,从第10周开始,中药组给予血尿安片灌胃,对照组和模型组以等量生理盐水灌胃,检测各组大鼠第10周、11周、12周末24 h尿蛋白定量,第12周末检测各组大鼠的尿肌酐、血肌酐,计算肌酐清除率。取肾组织观察病理变化,免疫荧光检测免疫球蛋白A(IgA);免疫组化检测肾小球系膜细胞增殖核抗原(PCNA)表达。结果:与模型组比较,血尿安片组大鼠24 h蛋白尿定量明显降低,肾小球肌酐清除率增多,肾组织损伤减轻,免疫荧光IgA数量及亮度均减低,肾小球内PCNA表达减少。结论:血尿安片能够降低IgAN大鼠的24 h蛋白定量,延缓肾功能下降,减轻肾脏病理损伤,机制或与抑制系膜细胞增殖有关。Objective:To observe the therapeutic effect of Xueniaoan Tablets on immunoglobulin A(IgA)nephropathy(IgAN)rats and its impact on glomerular mesangial cell proliferation.Methods:Thirty Sprague-Dawley(SD)rats were randomly divided into control group,model group,and Chinese medicine group using a random number table.An IgAN model was established,and from the 10th week,the Chinese medicine group was orally administered with Xueniaoan Tablets,while the control and model groups were administered an equal volume of physiological saline.The 24-hour urinary protein quantification was measured at the 10th,11th,and 12th weeks.At the end of the 12th week,urinary creatinine,blood creatinine,and creatinine clearance rate were measured in each group.Kidney tissue was collected to observe pathological changes,and immunofluorescence was used to detect IgA.Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen(PCNA)in glomerular mesangial cells.Results:Compared to the model group,the Xueniaoan Tablets group showed a significant decrease in 24-hour urinary protein levels,an increase in glomerular creatinine clearance rate,a reduction in kidney tissue damage,lower IgA quantity and fluorescence intensity,and a reduction in PCNA expression in the glomerulus.Conclusion:Xueniaoan Tablets can reduce 24-hour urinary protein levels,delay the decline in kidney function,and alleviate kidney pathological damage in IgAN rats.The mechanism may be related to the inhibition of mesangial cell proliferation.

关 键 词：IGA肾病 血尿安片 滋阴益肾 24 h蛋白定量 肌酐清除率 肾脏病理 肾小球系膜细胞增殖核抗原 荧光染色 

分 类 号：R256.5[医药卫生—中医内科学]