埃可病毒18型山东分离株遗传特征分析  

Genetic characterizations of echovirus 18 strains isolated from Shandong province,China

作　　者：刘棋 徐险峰 王敏 邢薇佳 史卫峰 李娟 LIU Qi;XU Xianfeng;WANG Min;XING Weijia;SHI Weifeng;LI Juan(Key Laboratory of Emerging Infectious Diseases in Universities of Shandong,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;Department of Pathogen Biology,School of Clinical and Basic Medicine Sciences,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;School of Biomedical Sciences,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;Department of Laboratory Medicine,Jinan Children's Hospital,Jinan 250022,China;School of Public Health,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Shanghai Institute of Virology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)山东省高等学校新发传染病重点实验室,山东济南250117 [2]山东第一医科大学(山东省医学科学院)临床与基础医学院(基础医学研究所)病原生物学系,山东济南250117 [3]山东第一医科大学(山东省医学科学院)生命科学学院,山东济南250117 [4]济南市儿童医院检验科,山东济南250022 [5]山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,山东济南250117 [6]上海交通大学医学院附属瑞金医院,上海200025 [7]上海交通大学医学院上海市病毒研究院,上海200025

出　　处：《山东第一医科大学(山东省医学科学院)学报》2025年第1期9-16,共8页Journal of Shandong First Medical University & Shandong Academy of Medical Sciences

基　　金：国家自然科学基金(82472254);山东省自然科学基金(ZR2024MH288);泰山学者工程专项(tsqn202211217);山东第一医科大学(山东省医学科学院)临床-基础联合创新团队项目(202407)。

摘　　要：目的分析山东3株埃可病毒18型(echovirus 18,E18)分离株的遗传特征,探明E18病毒的进化规律。方法采集2019-2020年山东省手足口病患者和疱疹性咽峡炎患者的血清样本,通过病毒分离、荧光定量检测和高通量测序,共获得3株E18分离株及其全基因组序列;利用MEGA 11和Bioedit软件进行E18病毒株VP1基因和全基因组序列的系统发育分析,采用Simplot软件和RDP软件进行重组分析,研究E18病毒株的遗传特征。结果3株E18分离株基因组序列长度分别为7367、7347和7383 bp,分离株间呈较高核苷酸相似性(97.92%~98.31%)和氨基酸相似性(99.11%~99.28%);3株E18分离株VP1基因位于我国主要流行基因型C基因亚型C2分支中,与我国流行的多数毒株均位于全基因组系统发育树的Group D分支。重组分析表明,3株E18分离株均为基因重组株,毒株基因组P2和P3区约4620~7200位点间与国内E30流行株A86/YN相似性较高。结论E18毒株在我国多地持续流行,3株山东分离株同国内多数E18株的VP1基因和全基因组均位于同一进化分支,它们所在C2基因型为国内VP1基因流行亚型,全基因组Group D为国内优势流行分支。E18毒株在P2和P3区与E30发生潜在型间重组,对E18的遗传多样性研究具有参考意义。Objective:To investigate the genetic characteristics of three echovirus 18(E18)strains isolated from Shandong Province,and to explore the evolutionary patterns of E18 strains.Methods:Serum samples were collected from patients with hand,foot and mouth disease and patients with herpangina in Shandong Province from 2019 to 2020.Through virus isolation,RT-qPCR amplification,and high-throughput sequencing,three E18 isolates were identified,and their whole-genome sequences were obtained.Phylogenetic trees were constructed based on the the full-length VP1 gene sequences and the complete genome sequences and of E18 strains using MEGA 11 and Bioedit.Recombination analysis was performed using Simplot and RDP.Results:Full-length genomes of the three E18 isolates were 7367 bp,7347 bp and 7383 bp in length,respectively.They showed high nucleotide(97.92%-98.31%)and amino acid(99.11%-99.28%)similarities.Phylogenetic analysis showed that the VP1 gene sequences of the three E18 strains were classified into the subgenotype C2,and their complete genome sequences clustered together with the majority of the Chinese strains within Group D,and their.Recombination analysis indicated that the three E18 isolates were potential recombinants with the P2 and P3 regions likely recombined from the Chinese E30 strain A86/YN(4620 bp-7200 bp).Conclusion:Our results highlight the constant circulation of E18 in China.The three E18 strains from Shandong Province were grouped together with the dominant E18 strains circulating in China.This suggests that they belong to the prevalent lineages:subgenotype C2 of the VP1 gene and the Group D of the complete genome.It also indicated that the potential inter-typic recombination had occurred between E18 and E30 in the P2 and P3 regions,providing insights into the genetic diversity of E18.

关 键 词：肠道病毒 埃可病毒18型 遗传特征分析 重组分析 

分 类 号：S85[农业科学—兽医学]