诃子、甘草与制草乌配伍对小鼠急性毒性的影响  

Effect of Compatibility of Prepared Aconitum kusnezoffii with Chebulae fructus and Glycyrrhiza uralensis on Acute Toxicity in Mice

作　　者：李林 李晗 陈腾飞 黄鹤 高云航 宋玲 侯红平[2] 张广平[2] 杨红[1] 叶祖光[2] LI Lin;LI Han;CHEN Tengfei;HUANG He;GAO Yunhang;SONG Ling;HOU Hongping;ZHANG Guangping;YANG Hong;YE Zuguang(School of Life Science,Liaoning Normal University,Dalian 116000,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]辽宁师范大学生命科学学院,大连116000 [2]中国中医科学院中药研究所,北京100700

出　　处：《世界中医药》2024年第24期3754-3760,共7页World Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1708200,2018YFC1708204);内蒙古自治区科技重大专项(2019ZD004)。

摘　　要：目的:通过观察不同剂量制草乌与诃子、甘草,以及3味药配伍对小鼠急性毒性的影响,从毒性角度探讨诃子和甘草是否对制草乌减毒。方法:采取单次灌胃给药的方式,以加权回归(Bliss)法测定半数致死数量(LD_(50))并分别对各剂量组进行一般症状观察、体质量和摄食量的测定。结果:制草乌组LD_(50):798.77 mg/kg,诃子-制草乌组LD_(50):927.27 mg/kg,甘草-制草乌组LD_(50):801.28 mg/kg,诃子-甘草-制草乌组LD_(50):1080.9 mg/kg。给药后第1天与空白组比较制草乌组小鼠体质量和摄食量显著降低,配伍组体质量和摄食量明显降低。第3天后小鼠体质量和摄食量与空白组比较差异无统计学意义。结论:诃子与甘草对制草乌具有一定减毒作用,其中减毒作用由强至弱依次为诃子-甘草-制草乌组>诃子-制草乌组>甘草-制草乌组。Objective:By observing the effect of compatibility of different doses of prepared Aconitum kusnezoffii with Chebulae fructus and Glycyrrhiza uralensis and the combination of the three drugs on acute toxicity in mice,this paper discussed whether Chebulae fructus and Glycyrrhiza uralensis can reduce the toxicity of prepared Aconitum kusnezoffii.Methods:After a single intragastric administration,the median lethal number(LD_(50))was determined by the Bliss method,and the general symptoms,body weight,and food intake were measured in each dose group.Results:LD_(50)in the prepared Aconitum kusnezoffii was 798.77 mg/kg,with that in the Chebulae fructus and prepared Aconitum kusnezoffii group being 927.27 mg/kg.LD_(50)in the Glycyrrhiza uralensis and prepared Aconitum kusnezoffii group was 801.28 mg/kg,and that in the Chebulae fructus,Glycyrrhiza uralensis,and prepared Aconitum kusnezoffii group was 1080.9 mg/kg.Compared with those in the blank group on the first day after administration,the body weight and food intake of mice in the prepared Aconitum kusnezoffii group were significantly reduced,and the body weight and food intake of mice in the compatibility group were obviously reduced.Conclusion:Chebulae fructus and Glycyrrhiza uralensis can reduce the toxicity of prepared Aconitum kusnezoffii.The toxicity reduction effect in descending order is Chebulae fructus,Glycyrrhiza uralensis,and prepared Aconitum kusnezoffii group>Chebulae fructus and prepared Aconitum kusnezoffii group>Glycyrrhiza uralensis and prepared Aconitum kusnezoffii group.

关 键 词：草乌 诃子 甘草 配伍 减毒 急性毒性 半数致死数量 扎冲十三味丸 

分 类 号：R285.5[医药卫生—中药学]