过表达SlWRKY41提高番茄幼苗抗旱性  

Overexpression of SlWRKY41 Improves the Tolerance of Tomato Seedlings to Drought

作　　者：刘洁[1] 王飞 陶婷 张玉静 陈浩婷 张瑞星 石玉[1] 张毅[1] LIU Jie;WANG Fei;TAO Ting;ZHANG Yu-jing;CHEN Hao-ting;ZHANG Rui-xing;SHI Yu;ZHANG Yi(College of Horticulture,Shanxi Agricultural University,Taigu 030801)

机构地区：[1]山西农业大学园艺学院,太谷030801

出　　处：《生物技术通报》2025年第2期107-118,共12页Biotechnology Bulletin

基　　金：山西省基础研究计划(20210302123366,202203021211263)。

摘　　要：【目的】探究SlWRKY41转录因子在番茄响应干旱胁迫中的作用,为进一步丰富番茄抗逆种质资源和选育抗旱品种提供理论依据。【方法】以野生型番茄‘Ailsa Craig’为材料,以其cDNA为模板克隆SlWRKY41基因,构建OE-SlWRKY41转基因番茄株系,对野生型和OE-SlWRKY41转基因番茄株系进行干旱处理,在处理第4、8天时对其进行表型观察和相关生理、生化指标的测定,并进行胁迫相关基因的表达分析。【结果】与野生型植株相比,干旱胁迫8 d后,OE-SlWRKY41转基因植株的叶片卷曲,萎蔫程度较轻,膜脂过氧化水平显著降低;气孔导度和蒸腾速率显著降低,净光合速率显著提高;根系形态指标整体呈现上升趋势,叶片相对含水量、抗氧化酶活性和渗透调节物质(可溶性糖和脯氨酸含量)显著增加,相对电导率显著降低;过表达SlWRKY41植株中的胁迫响应基因(SlSOD、SlPOD和SlP5CS1)显著上调。【结论】SlWRKY41能够响应干旱,过表达SlWRKY41能够提高番茄幼苗对干旱胁迫的耐受性。【Objective】To explore the role of SlWRKY41 transcription factor in response to drought stress in tomato,and to provide theoretical value and important basis for the enrichment of tomato germplasm resources and the selection and breeding of drought-resistant varieties of tomato.【Method】The experiment was conducted with wild-type tomato‘Ailsa Craig’as the material,and its cDNA was used as the template to clone the SlWRKY41 gene to construct the OE-SlWRKY41 transgenic tomato lines,and the wild-type and OE-SlWRKY41 transgenic tomato lines were subjected to drought stress treatments,and the phenotypic observations and related physiological and biochemical indexes were determined at the day 4 and 8 of the treatment.【Result】Compared with wild-type plants,after 8 d of drought stress,OESlWRKY41 transgenic plants had curled leaves with less wilting and significantly lower levels of membrane lipid peroxidation.Stomatal conductance and transpiration rates were significantly lower,and the net photosynthetic rate was significantly higher.The root morphology indexes showed an overall upward trend,and the leaf relative water content,antioxidant enzyme activities and osmotic regulating substances(soluble sugars and proline content)increased significantly,and relative conductivity decreased significantly.The stress response genes(SlSOD,SlPOD and SlP5CS1)in the plants overexpressing SlWRKY41 were significantly up-regulated.【Conclusion】SlWRKY41 respond to drought,and the overexpression of SlWRKY41 can improve the tolerance of tomato seedlings to drought stress.

关 键 词：番茄 转录因子 SlWRKY41 干旱胁迫 光合 抗氧化系统 渗透调节 

分 类 号：S64[农业科学—蔬菜学]