基于转录组分析植物激素对洋葱鳞茎膨大发育的调控机制  

Regulation Mechanism of Plant Hormones Related to Onion Bulb Enlargement and Development Based on Transcriptome Analysis

作　　者：李艳伟 杨妍妍[1] 孙亚玲[1] 霍雨猛[1] 王振宝[1] 刘冰江[1] LI Yan-wei;YANG Yan-yan;SUN Ya-ling;HUO Yu-meng;WANG Zhen-bao;LIU Bing-jiang(Institute of Vegetables,Shandong Academy of Agricultural Sciences,Ministry of Agriculture and Rural Affairs Key Laboratory of Huang Huai Protected Horticulture Engineering,Shandong Key Laboratory of Bulk Open-field Vegetable Breeding,Jinan 250100)

机构地区：[1]山东省农业科学院蔬菜研究所、农业农村部黄淮设施园艺工程重点实验室、山东省大宗露地蔬菜育种重点实验室,济南250100

出　　处：《生物技术通报》2025年第2期187-201,共15页Biotechnology Bulletin

基　　金：山东省农业科学院农业科技创新工程(CXGC2024F08);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-24-A-10)。

摘　　要：【目的】通过对洋葱发育不同阶段的鳞茎组织进行转录组比较分析,为研究植物激素在洋葱鳞茎形成过程中的分子调控机制提供依据。【方法】分别取洋葱出苗后33、47、61和75 d的鳞茎部位为试验材料,通过酶联免疫吸附法和气相色谱法检测,了解生长素、细胞分裂素、赤霉素3、脱落酸以及乙烯(前体ACC)含量在鳞茎膨大过程中的变化趋势;利用高通量测序技术进行转录组测序分析,从分子水平阐述植物激素对鳞茎发育的影响。【结果】从4个发育阶段的鳞茎中共检测到11814个差异表达基因(DEGs),其中共同DEGs有3311个;KEGG富集分析显示,不同发育阶段比较组DEGs均显著富集到“植物激素信号转导”通路,并对该通路上筛选到的36个主要DEGs进行表征;结合WGCNA分析每个模块与性状的r和P值,筛选到2个关键模块,通过转录因子预测,挖掘到3028个转录因子unigenes与13个植物激素信号结构基因unigenes共表达,转录因子unigenes功能注释来自46个基因家族,最多的为锌指蛋白(C_(2)C_(2)、C_(3)H),其次为bHLH、NAC、ERF基因家族。【结论】通过转录组分析揭示了植物激素信号转导途径参与洋葱鳞茎膨大发育的相关基因。【Objective】The comparative transcriptome of bulb tissues at different stages of onion development was analyzed to provide a basis for studying the molecular mechanism of plant hormones in onion(Allium cepa L.)bulb formation.【Method】Onion bulbs at 33,47,61 and 75 d after seedling emergence were collected as experimental materials.ELISA and gas chromatography were used to quantify the contents of auxin,cytokinin,gibberellin3,abscisic acid and ethylene(precursor ACC)during the bulb enlargement and development.Transcriptome sequencing was performed using high-throughput sequencing technology to elucidate the effects of plant hormones on bulb development at molecular level.【Result】A total of 11814 differentially expressed genes(DEGs)were detected from bulbs tissue at four developmental stages,of which 3311 were shared DEGs.KEGG enrichment analysis revealed that DEGs were significantly enriched into"plant hormone signal transduction"pathway in different comparison groups,and 36 major DEGs were screened and characterized in this pathway.Combining the r and P values of each module and trait in WGCNA(weighted gene co-expression network analysis)analysis,two key modules were screened out.Through transcription factor prediction,3028 transcription factor unigenes were found to be co-expressed with 13 plant hormone signaling genes.The functional annotation of transcription factor unigenes were derived from 46 gene families,the most important of which were zinc finger protein(C_(2)C_(2),C_(3)H),followed by bHLH,NAC and ERF gene families.【Conclusion】The genes related to plant hormone signal transduction pathway involved in onion bulb development is preliminarily revealed by transcriptome analysis.

关 键 词：洋葱 鳞茎膨大 转录组分析 植物激素 转录因子 

分 类 号：S63[农业科学—蔬菜学]