‘宁杞1号’响应NaCl胁迫相关基因的差异表达分析  

Differential Expression Analysis of Genes Related to NaCl Stress Response in Lycium barbarum‘Ningqi 1’

作　　者：李晶晶 胡进红 梁旺利 麻玉荣 梁文裕[1] 王玲霞[1] LI Jing-jing;HU Jin-hong;LIANG Wang-li;MA Yu-rong;LIANG Wen-yu;WANG Ling-xia(School of Life Sciences,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学生命科学学院,银川750021

出　　处：《生物技术通报》2025年第2期202-209,共8页Biotechnology Bulletin

基　　金：宁夏自然科学基金项目(2023AAC05024,2024AAC03097);国家自然科学基金项目(32301632)。

摘　　要：【目的】探究盐胁迫下‘宁杞1号’(Lycium barbarum Ningqi 1)信号转导途径相关基因差异表达规律,为深入解析‘宁杞1号’耐盐碱的分子机理奠定基础。【方法】基于转录组测序技术,对不同浓度NaCl胁迫下‘宁杞1号’叶片信号转导途径相关基因的差异表达进行分析,同时,对信号转导途径相关酶活性进行测定。【结果】(1)在0、100、200和300 mmol/L NaCl胁迫处理7 d时,从‘宁杞1号’叶片中共鉴定到3个信号转导途径和14个信号转导相关基因差异表达。(2)随着NaCl浓度的增加,CIPK6的相对表达量呈下降趋势;MAPKK2的相对表达量呈上升趋势;MAPKKK18和MAPK3的相对表达量呈先升后降的趋势,RTqPCR验证结果与RNA-seq测序结果基本一致。(3)随着NaCl浓度的增加,CIPK6、MAPKK2和MAPKKK18酶活性呈先升高后不变的趋势;MAPK3和PLD酶活性显著高于对照组;钙调素的含量随NaCl浓度增加而增加。【结论】‘宁杞1号’可能通过诱导磷脂酰肌醇、MAPK级联反应及依赖Ca^(2+)的SOS信号转导相关基因的差异表达响应NaCl胁迫,进而提高其耐盐性。【Objective】To explore the differential expression patterns of signal transduction pathways-related genes in Lycium barbarum Ningqi 1 under salt stress,and to lay a foundation for a deep understanding of the molecular mechanisms of L.barbarum‘Ningqi 1’tolerance to salinity.【Method】Transcriptome sequencing technique was used to analyze the signal transduction pathways-related differentially expressed genes in the leaves of L.barbarum Ningqi 1 under different concentrations of NaCl stress,and the changes in enzyme activities related to these pathways were also examined.【Result】1)A total of 14 genes related to three signal transduction pathways were differentially expressed in the leaves of L.barbarum Ningqi 1 after 7 d of treatment with 0,100,200 and 300 mmol/L NaCl stress.2)The relative expression of CIPK6 showed a downward trend,while that of MAPKK2 was in an upward trend.The relative expressions of MAPKKK18 and MAPK3 initially increased and then decreased with NaCl concentration increased.The RT-qPCR results were basically consistent with those obtained from RNA-seq.3)As the NaCl concentration increasing,the enzyme activities of CIPK6,MAPKK2 and MAPKKK18 initially rose and then unchanged.The enzyme activities of MAPK3 and PLD were significantly higher compared to the control group.The content of calmodulin increased with the rising NaCl concentration.【Conclusion】L.barbarum Ningqi 1 may respond to NaCl stress by inducing phosphatidylinositol,MAPK cascade and differential expression of Ca^(2+)dependent SOS signal transduction-related genes,and thus improve its tolerance to salt.

关 键 词：宁杞1号 NACL胁迫 信号转导 基因差异表达 

分 类 号：R73[医药卫生—肿瘤]