激素和非生物胁迫下荷花GH3基因家族的表达分析  

Expression Analysis of the GH3 Gene Family in Nelumbo nucifera under Hormonal and Abiotic Stresses

作　　者：匡健华 程志鹏 赵永晶 杨洁[1] 陈润乔 陈龙清 胡慧贞 KUANG Jian-hua;CHENG Zhi-peng;ZHAO Yong-jing;YANG Jie;CHEN Run-qiao;CHEN Long-qing;HU Hui-zhen(College of Landscape Architecture and Horticulture,Southwest Forestry University,Kunming 650224;Jiangbei District,Nanjing City,Jiangsu Province Rural Work Management Service Center,Nanjing 210031)

机构地区：[1]西南林业大学园林园艺学院,昆明650224 [2]江苏省南京市江北新区农村工作管理服务中心,南京210031

出　　处：《生物技术通报》2025年第2期221-233,共13页Biotechnology Bulletin

基　　金：国家自然科学基金地区科学基金项目(32360070);云南省重大科技专项计划(202202AE090028);云南省高层次人才引进计划高端外国专家专项(YNQR-GDWG-2020-001);云南省“兴滇英才计划”青年人才专项(YNQR-QNRC-2018-122);云南省农业基础联合面上项目(202101BD070001-124)。

摘　　要：【目的】探究荷花(Nelumbo nucifera)NnGH3家族成员的特征及其在非生物胁迫响应中的作用,为荷花抗逆新品种的选育提供新的关键候选基因资源。【方法】采用生物信息学方法从荷花全基因组中鉴定酰基酰胺合成酶(GH3)家族成员,对GH3基因编码的酰胺合成酶理化性质、染色体定位、三级结构预测、系统发育树、基因结构、共线性和表达模式等进行详细研究,并利用RT-qPCR技术探究NnGH3家族基因分别在低温(4℃)、厌氧(水淹)、盐害(300 mmol/L NaCl)及外源施加激素(0.1 mmol/L IAA、1 mmol/L JA和5 mmol/L SA)处理下的表达模式。【结果】从荷花基因组中鉴定出14个NnGH3家族成员,它们集中分布在5条染色体上,根据其在染色体的位置依次命名为NnGH3.1-3.14,其分子质量为365-630 aa,理论等电点均小于7,为酸性蛋白,亚细胞定位于细胞核和细胞质上;聚类分析表明GH3家族成员分为GroupⅠ和GroupⅡ两组,绝大多数成员含有3-4个外显子和GH3superfamily结构域。14个NnGH3基因中有2对基因存在共线性,与拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)GH3基因分别存在14和6对共线关系。顺式作用元件预测分析表明,该家族基因存在大量光、逆境和激素等响应元件。表达分析结果显示,NnGH3负向响应低温胁迫,绝大多数成员正向响应SA处理和盐胁迫,特异性响应IAA、JA处理及厌氧胁迫,其中GroupⅡ中的NnGH3.4和NnGH3.13基因以及GroupⅠ中的NnGH3.3基因在所有处理中表达量变化最为显著。【结论】在全基因组范围内从荷花中系统鉴定得到14个NnGH3家族成员,分为GroupⅠ和GroupⅡ两组,均含有GH3 superfamily结构域,为酸性蛋白。NnGH3家族基因在不同的外源激素和非生物胁迫条件下的表达具有特异性。【Objective】To characterize NnGH3 gene family in lotus(Nelumbo nucifera)and analyze their roles in abiotic stress,and to provide key candidate genetic resources for the breeding of new lotus varieties with stress resistance.【Method】Bioinformatics methods were used to identify the family members of acyl amides synthetase(GH3)from the whole genome of N.nucifera,and to conduct the detailed study on the phytochemical properties,chromosomal localization,tertiary structure prediction,systematic evolution,gene structure,collinearity relationships,and expression patterns of GH3 enzymes.RT-qPCR technology was applied to investigate the expression patterns of NnGH3 family genes under various stress conditions including low temperature(4℃),anaerobic conditions(submergence),salt stress(300 mmol/L NaCl),and exogenous hormone treatments(0.1 mmol/L IAA,1 mmol/L JA,and 5 mmol/L SA).【Result】The fourteen NnGH3 family members were identified from the lotus genome,distributed on 5 chromosomes,and named sequentially as NnGH3.1 to NnGH3.14 based on their chromosomal locations.Their molecular weights ranged from 365 to 630 amino acids,theoretical isoelectric points were all less than 7,and acidic proteins were localized in the nucleus and cytoplasm.Cluster analysis revealed two groups,Group I and GroupⅡ,within the GH3 family,with most members containing 3-4 exons and GH3 superfamily domains.Collinearity analysis indicated that 2 pairs of genes among the 14 lotus GH3 genes,14 pairs with(Arabidopsis thaliana)GH3 genes,and 6 pairs with(Oryza sativa)GH3 genes presented collinear relationships.Promoter cis-acting element analysis showed a lot of light-,stress-,and hormone-responsive elements within this family.Expression analysis demonstrated that NnGH3 genes negatively responded to low temperature stress,with most members positively responded to SA treatments and salt stress,and specifically responded to IAA and JA treatments as well as anaerobic stress.Notably,genes NnGH3.4 and NnGH3.13 from GroupⅡ,and NnGH3.3 from Group

关 键 词：荷花 GH3基因家族 生物信息学 植物激素 非生物胁迫 表达模式 

分 类 号：S682.32[农业科学—观赏园艺] Q943.2[农业科学—园艺学]