山新杨谷胱甘肽转移酶基因的生物信息学与表达模式分析  

Bioinformatics and Expression Pattern Analysis of Glutathione S-transferase in Populus davidiana×P.bolleana

作　　者：黄颖[1,2] 遇文婧 刘雪峰 刁桂萍[1] HUANG Ying;YU Wen-jing;LIU Xue-feng;DIAO Gui-ping(College of Forestry,Northeast Forestry University,Harbin 150040;Heilongjiang Institute of Forest Protection,Harbin 150040)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]黑龙江省森林保护研究所,哈尔滨150040

出　　处：《生物技术通报》2025年第2期248-256,共9页Biotechnology Bulletin

基　　金：中央高校基本科研业务费专项资金(2572022DS07);黑龙江省省属科研院所科研业务费(LKSB2024-2)。

摘　　要：【目的】分析山新杨谷胱甘肽转移酶(GST)基因的表达模式,为深入研究木本植物GST基因的功能提供基础。【方法】克隆山新杨GST基因并进行生物信息学分析,通过荧光定量PCR技术分析GST基因在植物激素及非生物胁迫下的表达模式。【结果】克隆了3个PdbGST基因家族成员,分别命名为PdbGST1、PdbGST2和PdbGST3,cDNA全长分别为678、660和690 bp,编码氨基酸分别为225、219和229个,形成的蛋白质均为稳定亲水酸性蛋白,且均定位于细胞质。同时,这3个蛋白质均具有谷胱甘肽转移酶的典型结构,且其启动子区域具有能够响应生物或非生物胁迫以及植物激素的元件。此外,这3个基因的表达不同程度地受外源植物激素或非生物胁迫诱导。【结论】3个PdbGST基因均能够不同程度地响应非生物胁迫或外源植物激素的诱导,可能参与到杨树对逆境胁迫的响应过程中。【Objective】To provide a basis for further study of the function of glutathione transferase(GST)gene in woody plants,the expression pattern of GST genes from Populus davidiana×P.bolleana were analyzed.【Method】The GST genes were cloned and analyzed by bioinformatics of Populus davidiana×P.bolleana.The expression pattern of GST genes under phytohormone and abiotic stresses were analyzed by real-time PCR.【Result】Three GST gene members from Populus davidiana×P.bolleana were cloned.The lengths of these genes were 678,660 and 690 bp,encoding amino acids of 225,219 and 229 aa,respectively.Meanwhile,all three proteins have the typical structure of glutathione transferase,and their promoter regions have elements that can respond to biotic or abiotic stresses as well as plant hormones.In addition,the expressions of these three genes were differently induced by exogenous phytohormones or abiotic stresses.【Conclusion】All the three PdbGSTs may respond to abiotic stresses or the induction of exogenous phytohormone,indicating that they may play roles in response to the stresses in poplar.

关 键 词：谷胱甘肽转移酶 生物信息学分析 基因表达模式 山新杨 

分 类 号：R73[医药卫生—肿瘤]