板栗SWEET基因家族成员的鉴定及表达分析  

Identification and Expression Analysis of Members of the SWEET Gene Family in Chinese Chestnut

作　　者：杨涌 袁国梅 康肖肖 刘亚明 王东升[1,2,3] 张海娥 YANG Yong;YUAN Guo-mei;KANG Xiao-xiao;LIU Ya-ming;WANG Dong-sheng;ZHANG Hai-e(College of Horticultural Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004;Engineering Research Center of Chestnut Industry Technology,Ministry of Education,Qinhuangdao 066004;Hebei Province Chestnut Industry Collaborative Innovation Center,Qinhuangdao 066004)

机构地区：[1]河北科技师范学院园艺科技学院,秦皇岛066004 [2]板栗产业技术教育部工程研究中心,秦皇岛066004 [3]河北省板栗产业协同创新中心,秦皇岛066004

出　　处：《生物技术通报》2025年第2期257-269,共13页Biotechnology Bulletin

基　　金：国家重点研发计划项目(2022YFD2200400);河北省重点研发计划项目(21326304D);河北科技师范学院博士启动基金项目(2023YB026)。

摘　　要：【目的】SWEET基因家族与果实中淀粉含量、糖含量密切相关,分析板栗SWEET基因家族在板栗果实成熟过程中的表达模式,为后续研究板栗SWEET基因家族在果实成熟过程中的作用奠定基础。【方法】利用生物信息学方法鉴定出7种壳斗科物种SWEET基因家族,对其进行系统进化树构建、各个成员同源性分析、基因复制类型分析等;同时分析板栗SWEET基因家族蛋白理化性质、基因结构、选择压力、蛋白结构等;利用转录组和RT-qPCR分析板栗SWEET基因家族成员在不同果实成熟时期的表达模式。【结果】7种壳斗科物种中共包含了129个SWEET基因家族成员,7种壳斗科物种分化之前共拥有16个SWEET基因家族成员。板栗SWEET基因家族共有19个,不均地分布在9条染色体和1条contig片段上;同时其蛋白拥有较为一致的Motif分布、保守结构域分布。顺势作用元件分析结果显示,大量的生长发育相关元件、激素响应元件以及胁迫响应元件存在于板栗SWEET基因家族启动子序列上。转录组和RT-qPCR分析显示,有9个板栗SWEET基因家族成员在板栗果实成熟过程中表达。其中CmSWEET1、CmSWEET3、Cm SWEET4、CmSWEET16及CmSWEET19随着果实成熟过程基因表达量下调;CmSWEET2及CmSWEET9随着果实成熟过程基因表达量上调;CmSWEET15与CmSWEET17随着果实成熟过程基因表达量先下调再上调。【结论】共鉴定了129个壳斗科物种SWEET基因家族成员,其中有19个板栗SWEET基因家族成员,CmSWEET15与CmSWEET17表达模式与可溶性糖变化模式相似,其可能调控板栗果实成熟过程中可溶性糖转运;CmSWEET1、CmSWEET2、CmSWEET3、CmSWEET4、CmSWEET9、CmSWEET16及Cm SWEET19表达模式与淀粉变化模式完全一致或完全相反,其可能调控板栗果实成熟过程中淀粉积累。【Objective】The SWEET gene family is closely related to the starch content and sugar content in the fruit.Focusing on the expression pattern of chestnut SWEET gene family during chestnut fruit ripening may lay the foundation for subsequent studies of the SWEET gene family function during fruit ripening in Chinese chestnut.【Method】Bioinformatics techniques was applied to identify the SWEET gene family of seven Fagaceae species.Phylogenetic tree was constructed on them,homology analysis of each member was performed,and gene duplication type analysis was performed,etc.The protein physicochemical properties,gene structure,selection pressure and protein structure of chestnut SWEET gene family were also analyzed.Transcriptome and RT-qPCR were used to analyze the expression patterns of chestnut SWEET gene family members during different fruit ripening period.【Result】A total of seven Fagaceae species contained the 129 members of SWEET gene family,and seven species had 16 SWEET gene family members before divergence.There were 19 chestnut SWEET gene families,which were unequally distributed across nine chromosomes and one contig segment.And their proteins had a relatively consistent Motif distribution and conserved domain distribution.The results of cis-element analysis showed that a large number of growth-related elements,hormone response elements and stress response elements were present on the promoter sequences of chestnut SWEET gene family.Transcriptome and RT-qPCR analyses revealed that nine members of the chestnut SWEET gene family were expressed during chestnut fruit ripening.Among them,CmSWEET1,CmSWEET3,CmSWEET4,CmSWEET16 and CmSWEET19 were downregulated with ripening,CmSWEET2 and CmSWEET9 were downregulated with ripening,and CmSWEET15 and CmSWEET17 were downregulated with ripening.【Conclusion】A total of 129 members of the Fagaceae species SWEET gene family are identified,including 19 members of the chestnut SWEET gene family.CmSWEET15 and CmSWEET17 expression pattern is similar to the change patter

关 键 词：板栗 壳斗科 基因家族 淀粉 可溶性糖 表达模式 生物信息学 

分 类 号：S66[农业科学—果树学]