溃疡性结肠炎小鼠结肠及免疫组织中四逆散药源性成分的LC-MS分析  

Analysis of xenobiotics in colon and immune tissues of ulcerative colitis mice after administration of Sini San by

作　　者：曹艳芳 汪雅莉 王安慧 陈永顺[1] 李思翰 冯凯 杨婉薇[3] 宋瑞[2] CAO Yanfang;WANG Yali;WANG Anhui;CHEN Yongshun;LI Sihan;FENG Kai;YANG Wanwei;SONG Rui(Shangqiu Medical College,Shangqiu 476000;School of Pharmacy,China Pharmaceutical University,Nanjing 210009;Department of Clinical Laboratory,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Jiangsu Province Academy of Traditional Chinese Medicine,Nanjing 210028,China)

机构地区：[1]商丘医学高等专科学校,商丘476000 [2]中国药科大学药学院,南京210009 [3]南京中医药大学附属中西医结合医院检验科,南京210028

出　　处：《中国药科大学学报》2025年第1期73-79,共7页Journal of China Pharmaceutical University

基　　金：江苏省自然科学基金项目(BK20161456);商丘医学高等专科学校开放课题(KFKT23002)。

摘　　要：免疫反应失调是目前公认的溃疡性结肠炎(UC)重要病理因素之一。本文在确证四逆散能显著改善葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的UC小鼠结肠炎症的基础上,采用高效液相色谱-离子阱飞行时间质谱(HPLC-IT-TOF-MS)技术系统研究了UC小鼠结肠及肠系膜淋巴结、脾脏、胸腺等免疫组织中的四逆散药源性成分。研究结果表明,除结肠外,四逆散中部分成分及其代谢物可分布于免疫组织,关联分析四逆散组成药味质量标准中指标性成分的含量限度,发现相对低丰度、弱响应成分如柴胡皂苷a、芍药苷、甘草酸等成分的质量具有向结肠和淋巴器官高效传递的特征,集中暴露在上述器官,极有可能是四逆散抗UC的药效物质来源。本研究的发现为后续确证成分组合物的药效并开展其改善UC的分子机制研究奠定了基础,也为四逆散临床合理应用提供科学依据,有望促进其中成药的二次开发。Dysregulation of immune response is currently recognized as one of the important pathological factors in ulcerative colitis(UC).Based on the confirmation that the Sini San(SNS)can significantly improve the colon inflammation induced by dextran sulfate sodium sulfate(DSS)in mice,the present work systematically studied the xenobiotics in the colon and mesenteric lymph nodes,spleen,and thymus of UC mice after administration of SNS by high-performance liquid chromatography-ion trap time-of-flight mass spectrometry(HPLC-IT-TOF-MS).The results showed that,in addition to the colon,some components and their metabolites in SNS could be distributed in immune tissues,and it was found that the quality of relatively low-abundance and weakly responsive components such as saikosaponin a,paeoniflorin,and glycyrrhizic acid had the characteristics of efficient transmission to the colon and lymphoid organs.These components were very likely to be the source of pharmacodynamic substances of SNS.The findings of this study lay a foundation for the study of the efficacy and molecular mechanism of the components against ulcerative colitis,and also provide a scientific basis for the rational clinical application of SNS,which is expected to promote the secondary development of its preparations.

关 键 词：四逆散 溃疡性结肠炎 结肠 免疫组织 药源性成分 

分 类 号：R917[医药卫生—药物分析学]