大麦芽碱对卵清蛋白诱导的大鼠变应性鼻炎的治疗作用及机制  

Therapeutic effect and mechanism of hordenine on ovalbumin-induced allergic rhinitis in rats

作　　者：李俊彦 刘涛[2] 孙芳 黄家辉 毛书振 姚静 LI Junyan;LIU Tao;SUN Fang;HUANG Jiahui;MAO Shuzhen;YAO Jing(Department of Clinical Pathology,School of Basic Medicine,Shandong Second Medical University,Weifang 261053;Department of Otolaryngology and Head&Neck Surgery,Affiliated Hospital of Jining Medical University,Jining 272067;School of Basic Medicine,Jining Medical University,Jining 272067;Department of Pharmacy,Shandong First Medical University,Jinan 250117;Jining Key Laboratory of Pharmacology,Jining 272067,China)

机构地区：[1]山东第二医科大学基础医学院临床病理系,潍坊261053 [2]济宁医学院附属医院耳鼻喉头颈外科,济宁272067 [3]济宁医学院基础医学院,济宁272067 [4]山东第一医科大学药学院,济南250117 [5]济宁市药理学重点实验室,济宁272067

出　　处：《中国药科大学学报》2025年第1期80-90,共11页Journal of China Pharmaceutical University

基　　金：山东省中医药科技项目(M-2022247);济宁医学院贺林院士新医学临床转化站科研基金项目(JYHL2021FMS13);济宁医学院实践教学教育研究计划项目(JYSJ2022B44)。

摘　　要：研究大麦芽碱对卵清蛋白(ovalbumin,OVA)诱导的大鼠变应性鼻炎(allergic rhinitis,AR)的治疗作用及相关机制。构建AR动物模型,采用HE染色和AB-PAS染色检测大麦芽碱对鼻黏膜病理损伤的改善情况。采用ELISA检测大麦芽碱对大鼠血清中OVA-sIgE及鼻黏膜组织上清液中IL-4的影响。采用免疫组化和Western blot实验检测大麦芽碱对Th1/Th2细胞平衡的影响。采用生物信息学进行通路预测,并通过体内及体外实验验证。实验结果表明,大麦芽碱可以减轻OVA诱导AR大鼠的行为学表现,缓解AR导致的鼻黏膜病理损伤,减少OVA-sIgE及IL-4的分泌。此外,大麦芽碱可以调节Th1/Th2平衡。生物信息学分析结果显示,大麦芽碱作用于AR的潜在作用通路为磷脂酰肌醇3(phosphoinositide 3 kinase,PI3K)/磷酸蛋白激酶(protein kinase B,Akt)信号通路。体内实验结果显示,模型组大鼠鼻黏膜中PI3K和p-Akt蛋白表达显著升高(P<0.01),经过大麦芽碱治疗后,其表达水平显著降低,体外细胞实验也验证了这一结果。本研究提示,大麦芽碱可能通过PI3K/Akt信号通路调节Th1/Th2细胞平衡,从而发挥缓解OVA诱导的变应性鼻炎的作用。To investigate the therapeutic effect and related mechanisms of hordenine on ovalbumin(OVA)-induced allergic rhinitis(AR)in rats,HE and AB-PAS staining were used to detect the improvement of pathological damage to the nasal mucosa induced by hordenine.ELISA was employed to detect the effect of hordenine on OVA-sIgE in serum and IL-4 in the nasal mucosa supernatant of rats.IHC and Western blot experiments were undertaken to examine the effect of hordenine on Th1/Th2 cell balance.Bioinformatics analysis was performed to predict pathways,which were verified by in vivo and in vitro experiments.The experimental results showed that hordenine could alleviate the behavioral manifestations of OVA-induced AR rats,alleviate nasal mucosal pathological damage caused by AR,and reduce the secretion of OVA-sIgE and IL-4.In addition,hordenine could regulate the Th1/Th2 balance.Bioinformatics analysis results showed that the potential pathway of action of hordenine on AR was the phosphoinositide 3 kinase(PI3K)/protein kinase B(Akt)signaling pathway.The in vivo experimental results showed that the expression of PI3K and p-Akt proteins in the nasal mucosa of the model group rats was significantly increased(P<0.01),and that the protein expression level was significantly decreased after the administration of hordenine,which was also confirmed by an in vitro experiment.This study suggests that hordenine may regulate Th1/Th2 cell balance through the PI3K/Akt signaling pathway,thereby exerting an alleviating effect on OVA-induced AR.

关 键 词：变应性鼻炎 大麦芽碱 PI3K/AKT信号通路 TH1/TH2细胞平衡 

分 类 号：R965[医药卫生—药理学]