黄芩素外用制剂对特应性皮炎的治疗作用及其机制  

Therapeutic effect and mechanism of the topical preparation of baicalein on atopic dermatitis

作　　者：王登 孙中英 郭青龙 李晰 白宇杰 魏立彬 何远 WANG Deng;SUN Zhongying;GUO Qinglong;LI Xi;BAI Yujie;WEI Libin;HE Yuan(School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing 211198;Nanjing Qinling Pharmaceutical Technology Co.,Ltd,Nanjing 210032;Shandong Provincial Key Laboratory of Polymorphic Drugs,Shandong Yikang Pharmaceutical Co.,Ltd.,Tengzhou 277500,China)

机构地区：[1]中国药科大学基础医学与临床药学学院,南京211198 [2]南京芩领医药科技有限公司,南京210032 [3]山东益康药业股份有限公司,山东省晶型药物重点实验室,滕州277500

出　　处：《中国药科大学学报》2025年第1期99-109,共11页Journal of China Pharmaceutical University

基　　金：国家自然科学基金项目(No.82002907);枣庄英才集聚工程;泰山产业领军人才工程;山东省中央引导地方科技发展资金项目(YDZX2023082)。

摘　　要：为评价黄芩素外用制剂对特应性皮炎的治疗效果,构建了卡泊三醇及2,4-二硝基-1-氟苯诱导的特应性皮炎样小鼠模型,利用皮肤组织染色等实验确定了黄芩素外用制剂的疗效。结果显示,其可缓解病变皮肤组织表皮增厚、修复受损的皮肤屏障蛋白,并抑制病变组织辅助性T细胞2(Th2)浸润及肥大细胞浸润和活化。通过网络药理学分析了黄芩素可通过干预多条特应性皮炎致病相关信号通路从而治疗特应性皮炎。随后,通过细胞机制实验确定黄芩素可降低角质形成细胞的炎症因子的mRNA水平并抑制NF-κB p65及STAT1蛋白的磷酸化,从而抑制炎症进程。研究表明,黄芩素外用制剂可通过下调角质形成细胞内NF-κB的磷酸化水平,从而降低角质形成细胞炎症因子的表达,而缓解小鼠特应性皮炎样症状,为黄芩素外用制剂对于特应性皮炎等皮肤炎症性疾病治疗提供思路和理论基础。To evaluate the therapeutic effect of baicalein topical preparation on atopic dermatitis,we first constructed two atopic dermatitis-like mouse models induced by calcipotriol and 1-fluoro-2,4-dinitrobenzene to assess their therapeutic effect with skin tissue staining and other experiments.It was found that topical preparation of baicalein could alleviate epidermal thickening of diseased skin tissues,repair damaged skin barrier proteins,and inhibit T helper 2 cells(Th2)infiltration and mast cell infiltration and activation in lesional sites.Cyberpharmacology was utilized to analyze whether baicalein could treat atopic dermatitis by interfering with multiple pathogenesis-associated pathways.Results indicated that baicalein reduced the mRNA levels of inflammatory factors and inhibited the phosphorylation of NF-κB p65 and STAT1 proteins in keratinocyte cells.Together,the topical preparation of baicalein may be effective in alleviating atopic dermatitis-like symptoms in mice by down-regulating the phosphorylation level of NF-κB in keratinocytes,thereby decreasing the expression of inflammatory factors in keratinocytes,which provides an idea and a theoretical basis for the topical preparation of baicalein for the treatment of inflammatory skin diseases such as atopic dermatitis.

关 键 词：特应性皮炎 黄芩素 外用制剂 炎症因子 NF-κB p65 

分 类 号：R967[医药卫生—药理学]