牛昏睡嗜血杆菌的分离鉴定及其应用研究  

Isolation and Characterization of Haemophilus somnus from Cattle and Its Application Study

作　　者：刘玉梅 张彦婷 赵苏苏 赵利平 刘东霞 辛颖 方建国 杨晓婷 莫绍江 陈学飞 张鸣华 范文霞 赵丽霞 关平原 LIU Yu-mei;ZHANG Yan-ting;ZHAO Su-su;ZHAO Li-ping;LIU Dong-xia;XIN Ying;FANG Jian-guo;YANG Xiao-ting;MO Shao-jiang;CHEN Xue-fei;ZHANG Ming-hua;FAN Wen-xia;ZHAO Li-xia;GUAN Ping-yuan(The Spirit Jinyu Biological Pharmaceutical Co.,Ltd.,Hohhot 010111,China;National Engineering Laboratory for Veterinary Vaccine,Hohhot 010111,China)

机构地区：[1]金宇保灵生物药品有限公司,呼和浩特010111 [2]兽用疫苗国家工程实验室,呼和浩特010111

出　　处：《中国兽药杂志》2025年第2期1-7,共7页Chinese Journal of Veterinary Drug

基　　金：内蒙古自治区科技重大专项牛羊支原体肺炎和病毒性呼吸道疾病的防控技术研发与示范(2021ZD0013)。

摘　　要：为明确国内流行牛昏睡嗜血杆菌病病原特性,将临床采集牛昏睡嗜血杆菌病诊断阳性病料划线接种于脑心浸液酵母浸提膏血琼脂固体培养基,经多代次最适固液培养基纯化分离培养,获得稳定传代的目标菌株,经8~12 h液体培养基培养,其菌数可达3×10^(9) CFU/mL,对小鼠的最小致死剂量为4×10^(8) CFU,致病性较强。将培养菌液制备成灭活疫苗免疫小鼠后,能够针对流行的昏睡嗜血杆菌致病菌株产生80%以上免疫保护效果,且对小鼠安全性良好。该菌株的成功分离对牛昏睡嗜血杆菌病疫苗的研制具有促进作用。In order to clarify the pathogen characteristics of the Haemophilus somnus disease in China,the clinical samples with positive diagnosis of Haemophilus somnus were inoculated on the solid medium of Brain Heart Extraction Yeast Extraction Ointment Blood Agar.The target strains were purified and cultured on the most suitable solid-liquid medium for many generations.After 8~12 hours of liquid medium culture,the number of bacteria could reach 3×10^(9) CFU/mL,and the minimum lethal dose for all mice was 4×10^(8) CFU,which is highly pathogenic.After immunizing mice with inactivated vaccine prepared from the culture liquid,it can produce more than 80%immune protection effect against the epidemic pathogenic strain of Haemophilus somnus,and the safety is good.The successful isolation of this strain will play a positive effect on the development of a vaccine against bovine Hemophilus sommus disease.

关 键 词：昏睡嗜血杆菌 分离 培养 PCR鉴定 致病力 

分 类 号：S852.61[农业科学—基础兽医学]