A review on recent taxonomic updates of gut bacteria associated with social bees,with a curated genomic reference database  

作　　者：Chengfeng Yang Jiawei Hu Qinzhi Su Zijing Zhang Yating Du Jieni Wang Huihui Sun Benfeng Han Junbo Tang Lizhen Guo Hu Li Wanzhi Cai Hao Zheng Xin Zhou Xue Zhang 

机构地区：[1]Department of Entomology,College of Plant Protection,China Agricultural University,Beijing,China [2]Sanya Institute of China Agricultural University,Hainan Province,China [3]College of Food Science and Nutritional Engineering,China Agricultural University,Beijing,China [4]Beijing Laboratory of Biomedical Materials,Department of Geriatric Dentistry,Peking University School and Hospital of Stomatology,Beijing,China [5]Hebei Key Laboratory of Animal Physiology,Biochemistry and Molecular Biology,College of Life Sciences,Hebei Normal University,Shijiazhuang,China

出　　处：《Insect Science》2025年第1期2-23,共22页昆虫科学(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(32370550 and 32000346)to Xue Zhang;the program from the Sanya Institute of China Agricultural University(SYND-2021-30);by 2115 Talent Development Program of China Agricultural University grants to Xin Zhou。

摘　　要：Honeybees and bumblebees play a crucial role as essential pollinators.The special gut microbiome of social bees is a key factor in determining the overall fitness and health of the host.Although bees harbor relatively simple microbial communities at the genus level,recent studies have unveiled significant genetic divergence and variations in gene content within each bacterial genus.However,a comprehensive and refined genomics-based taxonomic database specific to social bee gut microbiomes remains lacking.Here,we first provided an overview of the current knowledge on the distribution and function of social bee gut bacteria,as well as the factors that influence the gut population dynamics.We then consolidated all available genomes of the gut bacteria of social bees and refined the species-level taxonomy,by constructing a maximum-likelihood core genome phylogeny and calculating genome-wide pairwise average nucleotide identity.On the basis of the refined species taxonomy,we constructed a curated genomic reference database,named the bee gut microbe genome sequence database(BGM-GDb).To evaluate the species-profiling performance of the curated BGM-GDb,we retrieved a series of bee gut metagenomic data and inferred the species-level composition using metagenomic intra-species diversity analysis system(MIDAS),and then compared the results with those obtained from a prebuilt MIDAS database.We found that compared with the default database,the BGM-GDb excelled in aligned read counts and bacterial richness.Overall,this high-resolution and precise genomic reference database will facilitate research in understanding the gut community structure of social bees.

关 键 词：bacterial species BGM-GDb corbiculate bees gut microbiome phylogeny PHYLOTYPE 

分 类 号：R57[医药卫生—消化系统]