The circadian clock affects starvation resistance through the pentose phosphate pathway in silkworm,Bombyx mori  

作　　者：Tai-Ming Dai Jian-Feng Qiu Cheng Luo Wen-Zhao Cui Kai Liu Jiang-Lan Li Ruji Peng Yang-Hu Sima Shi-Qing Xu 

机构地区：[1]School of Life Sciences,Suzhou Medical College of Soochow University,Suzhou,Jiangsu Province,China [2]Institute of Agricultural Biotechnology&Ecology(IABE),Soochow University,Suzhou,Jiangsu Province,China

出　　处：《Insect Science》2025年第1期55-68,共14页昆虫科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(Grant No.32172794 to S.-Q.X.and 32302817 to J.-F.Q.);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD to S.-Q.X.);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX23_3259 to R.-J.P)。

摘　　要：Disruption of the circadian clock can affect starvation resistance,but the molecular mechanism is still unclear.Here,we found that starvation resistance was significantly reduced in the core gene BmPer deficient mutant silkworms(Per^(−/−)),but the mutant's starvation resistance increased with larval age.Under natural physiological conditions,the weight of mutant 5th instar larvae was significantly increased compared to wild type,and the accumulation ability of triglycerides and glycogen in the fat bodies was upregulated.However,under starvation conditions,the weight consumption of mutant larvae was increased and cholesterol utilization was intensified.Transcriptome analysis showed that beta-oxidation was significantly upregulated under starvation conditions,fatty acid synthesis was inhibited,and the expression levels of genes related to mitochondrial function were significantly changed.Further investigations revealed that the redox balance,which is closely related to mitochondrial metabolism,was altered in the fat bodies,the antioxidant level was increased,and the pentose phosphate pathway,the source of reducing power in cells,was activated.Our findings suggest that one of the reasons for the increased energy burden observed in mutants is the need to maintain a more robust redox balance in metabolic tissues.This necessitates the diversion of more glucose into the pentose phosphate pathway to ensure an adequate supply of reducing power.

关 键 词：Bombyx mori circadian clock oxidative stress pentose phosphate pathway starvationresistance 

分 类 号：Q96[生物学—昆虫学]