Dual-role regulator of a novel miR-3040 in photoperiod-mediated wing dimorphism and wing development in green peach aphid  

作　　者：Yun-Hui Zhang Xin Qian Xin Zong Shi-Heng An Shuo Yan Jie Shen 

机构地区：[1]College of Plant Protection,Henan Agricultural University,Zhengzhou,China [2]Department of Plant Biosecurity,College of Plant Protection,China Agricultural University,Beijing,China

出　　处：《Insect Science》2025年第1期80-94,共15页昆虫科学(英文版)

基　　金：the National Natural Science Foundation of China(32030012);National Key Research and Development Program of the Ministry of Science and Technology(2022YFD1401800).

摘　　要：Wing dimorphism is regarded as an important phenotypic plasticity involved in the migration and reproduction of aphids.However,the signal transduction and regulatory mechanism of wing dimorphism in aphids are still unclear.Herein,the optimal environmental conditions were first explored for inducing winged offspring of green peach aphid,and the short photoperiod was the most important environmental cue to regulate wing dimorphism.Compared to 16 L:8 D photoperiod,the proportion of winged offspring increased to 90%under 8 L:16 D photoperiod.Subsequently,5 differentially expressed microRNAs(miRNAs)in aphids treated with long and short photoperiods were identified using small RNA sequencing,and a novel miR-3040 was identified as a vital miRNA involved in photoperiod-mediated wing dimorphism.More specifically,the inhibition of miR-3040 expression could reduce the proportion of winged offspring induced by short photoperiod,whereas its activation increased the proportion of winged offspring under long photoperiod.Meanwhile,the expression level of miR-3040 in winged aphids was about 2.5 times that of wingless aphids,and the activation or inhibition of miR-3040 expression could cause wing deformity,revealing the dual-role regulator of miR-3040 in wing dimorphism and wing development.In summary,the current study identified the key environmental cue for wing dimorphism in green peach aphid,and the first to demonstrate the dual-role regulator of miR-3040 in photoperiod-mediated wing dimorphism and wing development.

关 键 词：miR-3040 Myzus persicae PHOTOPERIOD wing development wing dimorphism 

分 类 号：Q96[生物学—昆虫学]