Genome-wide identification of yellow gene family in Hermetia illucens and functional analysis of yellow-y by CRISPR/Cas9  

作　　者：Yongcheng Dong Xiaomiao Xu Lansa Qian Zongqing Kou Awawing A.Andongma Lijun Zhou Yongping Huang Yaohui Wang 

机构地区：[1]Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Insttutes,College of Plant Protection,Anhui Agricultural University,Hefei,China [2]Chinese Academy of Sciences(CAS)Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,CAS,Shanghai,China [3]Insect and Parasite Ecology Group,Lancaster Environment Centre,Lancaster University,Lancaster,UK [4]School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai,China

出　　处：《Insect Science》2025年第1期115-126,共12页昆虫科学(英文版)

基　　金：funded by a Talent Research Project of Anhui Agricultural University(rc342219);by an Anhui Province Key R&D Project(202204c06020020).

摘　　要：The yellow gene family plays a crucial role in insect pigmentation.It has potential for use as a visible marker gene in genetic manipulation and transgenic engineering in several model and non-model insects.Sadly,yellow genes have rarely been identified in Stratiomyidae species and the functions of yellow genes are relatively unknown.In the present study,we first manually annotated and curated 10 yellow genes in the black soldier fly(BSF),Hermetia illucens(Stratiomyidae).Then,the conserved amino acids in the major royal jelly proteins(MRJPs)domain,structural architecture and phylogenetic relationship of yellow genes in BSF were analyzed.We found that the BSF yellow-y,yellow-c and yellow-f genes are expressed at all developmental stages,especially in the prepupal stage.Using the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system,we successfully disrupted yellow-y,yellow-c and yellow-f in the BSF.Consequently,the mutation of yellow-y clearly resulted in a pale-yellow body color in prepupae,pupae and adults,instead of the typical black body color of the wild type.However,the mutation of yellow-c or yellow-f genes did not result in any change in color of the insects,when compared with the wild type.Our study indicates that the BSF yellow-y gene plays a role in body pigmentation,providing an optimal marker gene for the genetic manipulation of BSF.

关 键 词：CRISPR-Cas9 Hermetia illucens PIGMENTATION yellow family yellow-y 

分 类 号：Q78[生物学—分子生物学]