Systematic analysis of innate immune-related genes in the silkworm:Application to antiviral research  

作　　者：Junming Xia Ruoxuan Peng Shigang Fei Mian Muhammad Awais Wenxuan Lai Yigui Huang Hailin Wu Yue Yu Lingying Liang Luc Swevers Jingchen Sun Min Feng 

机构地区：[1]Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou,China [2]Insect Molecular Genetics and Biotechnology,National Center for Scientific Research Demokritos,Institute of Biosciences and Applications,Athens,Greece

出　　处：《Insect Science》2025年第1期151-171,共21页昆虫科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(32372945);Guangzhou Science and Technology Plan(202201010369);Natural Science Foundation of Guangdong Basic and Applied Basic Research Fund(2022A1515012657);South China Agricultural University high-level talent launch project.

摘　　要：The silkworm,a crucial model organism of the Lepidoptera,offers an excellent platform for investigating the molecular mechanisms underlying the innate immune response of insects toward pathogens.Over the years,researchers worldwide have identified numerous immune-related genes in silkworms.However,these identified silkworm immune genes are not well classified and not well known to the scientific community.With the availability of the latest genome data of silkworms and the extensive research on silkworm immunity,it has become imperative to systematically categorize the immune genes of silkworms with different database IDs.In this study,we present a meticulous organization of prevalent immune-related genes in the domestic silkworm,using the SilkDB 3.0 database as a reliable source for updated gene information.Furthermore,utilizing the available data,we classify the collected immune genes into distinct categories:pattern recognition receptors,classical immune pathways,effector genes and others.In-depth data analysis has enabled us to predict some potential antiviral genes.Subsequently,we performed antiviral experiments on selected genes,exploring their impact on Bombyx mori nucleopolyhedrovirus replication.The outcomes of this research furnish novel insights into the immune genes of the silkworm,consequently fostering advancements in the field of silkworm immunity research by establishing a comprehensive classification and functional understanding of immune-related genes in the silkworm.This study contributes to the broader understanding of insect immune responses and opens up new avenues for future investigations in the domain of host–pathogen interactions.

关 键 词：antiviral defense BMNPV Bombyx mori innate immunity SilkDB 3.0 

分 类 号：Q96[生物学—昆虫学]