A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer,Ostrinia furnacalis  

作　　者：Er-Tao Li Jia-Yue Ji Wei-Jie Kong Dong-Xu Shen Cai Li Chun-Ju An 

机构地区：[1]Deparment of Entomology,College of Plant Protection,China Agricultural University,Bejing,China [2]Pomology Institute,Shanxi Agricultural University,Jinzhong,Shanxi Province,China [3]Jiangsu Key Laboratory of Sericultural Biology and Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu Province,China

出　　处：《Insect Science》2025年第1期172-192,共21页昆虫科学(英文版)

基　　金：supported by the National Key Research and Development Program of China(2019YFE0120400);National Natural Science Foundation of China(31672361).

摘　　要：C-type lectins(CTLs)act as pattern recognition receptors(PRRs)to initiate the innate immune response in insects.A CTL with dual carbohydrate recognition domains(CRDs)(named immulectin-4[IML-4])was selected from the Ostrinia furnacalis transcriptome dataset for functional studies.We cloned the full-length complementary DNA of O.furnacalis IML-4(OfIML-4).It encodes a 328-residue protein with a Glu-Pro-Asn(EPN)and Gln-Pro-Asp(QPD)motifs in 2 CRDs,respectively.OfIML-4 messenger RNA levels increased significantly upon the bacterial and fungal infection.Recombinant OfIML-4(rIML-4)and its individual CRDs(rCRD1 and rCRD2)exhibited the binding ability to various microorganisms including Escherichia coli,Micrococcus luteus,Pichia pastoris,and Beauveria bassiana,and the cell wall components including lipopolysaccharide from E.coli,peptidoglycan from M.luteus or Bacillus subtilis,and curdlan from Alcaligenes faecalis.The binding further induced the agglutination of E.coli,M.luteus,and B.bassiana in the presence of calcium,the phagocytosis of Staphylococcus aureus by the hemocytes,in vitro encapsulation and melanization of nickel-nitrilotriacetic acid beads,and a significant increase in phenoloxidase activity of plasma.In addition,rIML-4 significantly enhanced the phagocytosis,nodulation,and resistance of O.furnacalis to B.bassiana.Taken together,our results suggest that OfIML-4 potentially works as a PRR to recognize the invading microorganisms,and functions in the innate immune response in O.furnacalis.

关 键 词：cellular immunity C-type lectin humoral immunity immulectin Ostrinia furnacalis pattern recognition receptor(PRR) 

分 类 号：TG1[金属学及工艺—金属学]