Strong resistance toβ-cyfluthrin in a strain of the beetle Alphitobius diaperinus:a de novo transcriptome analysis  

作　　者：Gwenola Gouesbet David Renault Stéphane APDerocles HervéColinet 

机构地区：[1]CNRS,ECOBIO(Ecosystèmes,Biodiversité,Évolution)—UMR 6553,University of Rennes,Rennes,France [2]Institut Universitaire de France,1 rue Descartes,CEDEX 05,Paris,France

出　　处：《Insect Science》2025年第1期209-226,共18页昆虫科学(英文版)

摘　　要：The lesser mealworm,Alphitobius diaperinus,is an invasive tenebrionid beetle and a vector of pathogens.Due to the emergence of insecticide resistance and consequent outbreaks that generate significant phytosanitary and energy costs for poultry farmers,it has become a major insect pest worldwide.To better understand the molecular mechanisms behind this resistance,we studied a strain of A.diaperinus from a poultry house in Brittany that was found to be highly resistant to theβ-cyfluthrin.The strain survivedβ-cyfluthrin exposures corresponding to more than 100 times the recommended dose.We used a comparative de novo RNA-Seq approach to explore genes expression in resistant versus sensitive strains.Our de novo transcriptomic analyses showed that responses toβ-cyfluthrin likely involved a whole set of resistance mechanisms.Genes related to detoxification,metabolic resistance,cuticular hydrocarbon biosynthesis and proteolysis were found to be constitutively overexpressed in the resistant compared to the sensitive strain.Follow-up enzymatic assays confirmed that the resistant strain exhibited high basal activities for detoxification enzymes such as cytochrome P450 monooxygenase and glutathione-S-transferase.The in-depth analysis of differentially expressed genes suggests the involvement of complex regulation of signaling pathways.Detailed knowledge of these resistance mechanisms is essential for the establishment of effective pest control.

关 键 词：Alphitobius diaperinus de novo transcriptome insecticide resistance P450 xenobiotic metabolism 

分 类 号：X59[环境科学与工程—环境工程]