Magnaporthe oryzae infection triggers rice resistance to brown planthopper through the influence of jasmonic acid on the flavonoid biosynthesis pathway  

作　　者：Su Chen Zhihuan Tao Yanjie Shen Rui Yang Siyuan Yan Zixu Chen Bo Sun Xiaofang Yang 

机构地区：[1]Precision Medicine Laboratory for Chronic Non-communicable Diseases of Shandong Province,Institute of Precision Medicine,Jining Medical University,Jining,Shandong Province,China [2]Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,China [3]College of Medical Engineering&The Key Laboratory for Medical Functional Nanomaterials,Jining Medical University,Jining,Shandong Province,China [4]Institute of Bioengineering,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou,China

出　　处：《Insect Science》2025年第1期243-259,共17页昆虫科学(英文版)

基　　金：supported by the Faculty Startup Fund for CS from Jining Medical University and by Shandong Provincial Natural Science Foundation(ZR2023QC309).

摘　　要：In agroecosystems,plants are constantly exposed to attack from diverse herbivorous insects and microbes,and infestation with one species may change the plant defense response to other species.In our investigation of the relationships among rice plants,the brown planthopper Nilaparvata lugens(Stål)and the rice blast fungus Magnaporthe oryzae,we observed a significant increase in the resistance of rice treated with rice blast to N.lugens,as evidenced by improved plant survival rates in a small population resistance study.Subsequent transcriptome data analysis revealed that the rice blast fungus can induce the expression of genes in the jasmonic acid(JA)and flavonoid pathways.Similar to the flavonoid pathway,the JA pathway also contains 2 types of genes that exhibit similar and opposite trends in response to N.lugens and rice blast.Among these genes,the osjaz1 mutant and the osmyc2 mutant were phenotypically confirmed to positively and negatively regulate rice resistance to N.lugens and rice blast,respectively.Subsequent mass spectrometry and quantification experiments showed that the exogenous application of methyl jasmonate(MeJA)can induce the accumulation of eriodictyol,naringenin and quercetin,as well as the expression of OsF3H,Os4CL5 and OsCHI in the flavonoid pathway.This suggests a close connection between the JA pathway and the flavonoid pathway.However,OsF3'H,which negatively regulates rice resistance to N.lugens and rice blast,did not show increased expression.Phenotypic and molecular experiments confirmed that OsMYC2 can bind to and inhibit the expression of OsF3'H,thus revealing the mechanism of rice resistance to N.lugens after treatment with rice blast.These findings will deepen our understanding of the interactions among rice,N.lugens and rice blast.

关 键 词：flavonoid jasmonic acid Nilaparvata lugens RICE rice blast 

分 类 号：S51[农业科学—作物学]