Antibiotic exposure alters the honeybee gut microbiota and may interfere with the honeybee behavioral caste transition  

作　　者：Zijing Zhang Xiaohuan Mu Qina Cao Yifan Zhai Li Zheng Yan Liu Hao Zheng Xue Zhang 

机构地区：[1]Hebei Key Laboratory of Animal Physiology,Biochemistry and Molecular Biology,Hebei Collaborative Innovation Center for Eco-Environment,College of Life Sciences,Hebei Normal University,Shijiazhuang,China [2]College of Food Science and Nutritional Engineering,China Agricultural University,Bejing,China [3]Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan,China [4]Key Laboratory of Natural Enemies Insects,Ministry of Agriculture and Rural Affairs,Jinan,China [5]Department of Entomology,College of Plant Protection,China AgriculturalUniversity,Beijing,China

出　　处：《Insect Science》2025年第1期260-276,共17页昆虫科学(英文版)

基　　金：funded by National Natural Science Foundation of China Projects 32370550 and 32300385;the Science Foundation of Hebei Normal University(L2023B21).

摘　　要：Behavioral division is essential for the sustainability and reproduction of honeybee populations.While accumulating evidence has documented that antibiotic exposure interferes with bee behavioral divisions,how the gut microbiome,host physiology,and genetic regulation are implicated in this process remains understudied.Here,by constructing single-cohort colonies,we validated that the gut microbiota varied in composition between age-matched nurse and forager bees.Perturbing the gut microbiota with a low dose of antibiotic retained the gut bacterial size,but the structure of the microbial community continuously diverged from the control group after antibiotic treatment.Fewer foragers were observed in the antibiotic groups in the field experiment.A combinatorial effect of decreased gut metabolic gene repertoires,reduced brain neurotransmitter titers,and downregulated brain immune genes could potentially be related to behavioral tasks transition delay.This work indicates that the disturbance to both the gut microbiome and host physiologies after antibiotic exposure may have implications on social behavior development,highlighting the need for further research focusing on antibiotic pollution threatening the honeybee population's health.

关 键 词：antibiotic resistance Apis mellifera behavioral development immune genes 

分 类 号：R57[医药卫生—消化系统]