A comprehensive framework for the delimitation of species within the Bemisia tabaci cryptic complex,a global pest-species group  

作　　者：Hua-Ling Wang Teng Lei Xiao-Wei Wang Stephen Cameron Jesús Navas-Castillo Yin-Quan Liu M.N.Maruthi Christopher A.Omongo Hélène Delatte Kyeong-Yeoll Lee Renate Krause-Sakate James Ng Susan Seal Elvira Fiallo-Olivé Kathryn Bushley John Colvin Shu-Sheng Liu 

机构地区：[1]College of Forestry,Hebei Agricultural University,Baoding,Hebei Province,China [2]The Ministry of Agriculture Key Laboratory of Molec ular Biology of Crop Pathogens and Insects,Institute of Insect Sciences,Zhejiang University,Hangzhou,China [3]Natural Resources Institute,University of Greenwich,Kent,UK [4]College of Life Sciences,Taizhou University,Taizhou,Zhejiang Province,China [5]Department of Entomology,Purdue University,West Lafayette,IN,USA [6]Instituto de Hortofruticultura Subtropical y Mediterranea"La Mayora"(IHSMUMA-CsiC),Consejo Superior de Investigaciones Cientificas,Malaga,Spain [7]National Crops Resources Research Institute,Kampala,Uganda [8]CIRAD,UMR PVBMT CIRAD,Pole de Protection des Plantes,Saint-Pierre,France [9]School of Applied Biosciences,Kyungpook National University,Daegu,Republic of Korea [10]uNESP,Faculdade de Ciencias Agronomicas,Botucatu,Brazil [11]Department of Microbiology and Plant Pathology,University of California,Riverside,CA [12]uSDA Agricultural Research Service,17123,Emerging Pests and Pathogens Research Unit,Ithaca,NY,USA

出　　处：《Insect Science》2025年第1期321-342,共22页昆虫科学(英文版)

基　　金：Peter Sseruwagi and Joseph Ndunguru,Mikocheni Agriculture Research Institute,Tanzania,for collecting samples from Uganda.J.N.C.is supported by grants(AGL2013-48913-C2-1-R and AGL2016-75819-C2-2-R)from the Ministerio de Economia y Competitividad(MINECO,Spain);co-financed by ERDF.E.F.O.is a recipient of a"Juan de la Cierva-Incorporacion"postdoctoral contract from MINECO.

摘　　要：Identifying cryptic species poses a substantial challenge to both biologists and naturalists due to morphological similarities.Bemisia tabaci is a cryptic species complex containing more than 44 putative species;several of which are currently among the world's most destructive crop pests.Interpreting and delimiting the evolution of this species complex has proved problematic.To develop a comprehensive framework for species delimitation and identification,we evaluated the performance of distinct data sources both individually and in combination among numerous samples of the B.tabaci species complex acquired worldwide.Distinct datasets include full mitogenomes,single-copy nuclear genes,restriction site-associated DNA sequencing,geographic range,host speciation,and reproductive compatibility datasets.Phylogenetically,our well-supported topologies generated from three dense molecular markers highlighted the evolutionary divergence of species of the B.tabaci complex and suggested that the nuclear markers serve as a more accurate representation of B.tabaci species diversity.Reproductive compatibility datasets facilitated the identification of at least 17 different cryptic species within our samples.Native geographic range information provides a complementary assessment of species recognition,while the host range datasets provide low rate of delimiting resolution.We further summarized different data performances in species classification when compared with reproductive compatibility,indicating that combination of mtCOI divergence,nuclear markers,geographic range provide a complementary assessment of species recognition.Finally,we represent a model for understanding and untangling the cryptic species complexes based on the evidence from this study and previously published articles.

关 键 词：Bemisia tabaci cryptic species PHYLOGENETICS RAD-Seq reproductive compatibility WHITEFLIES 

分 类 号：Q96[生物学—昆虫学]