自凝聚丝素蛋白微纳米纤维膜的制备及其力学增强  

Fabrication and mechanical reinforcement of self-coagulated regenerated silk fibroin micro-nanofiber membranes

作　　者：詹克静 杨鑫 张应龙 张昕[1,2] 潘志娟 ZHAN Kejing;YANG Xin;ZHANG Yinglong;ZHANG Xin;PAN Zhijuan(College of Textile and Clothing Engineering,Soochow University,Suzhou,Jiangsu 215021,China;National Engineering Laboratory for Modern Silk,Soochow University,Suzhou,Jiangsu 215123,China)

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215021 [2]苏州大学现代丝绸国家工程实验室,江苏苏州215123

出　　处：《纺织学报》2025年第2期10-19,共10页Journal of Textile Research

基　　金：中国博士后科学基金项目(2024M752322);江苏省卓越博士后计划项目(2023ZB420);江苏省丝绸工程重点实验室开放课题资助项目(KJS2314)。

摘　　要：再生丝素蛋白(RSF)微介观尺度的重构,是提升RSF材料力学性能的有效手段。为大力拓展RSF在生物医用领域的应用,通过模拟蚕腺体内的微环境,利用盐离子体系诱导RSF溶液产生液-液相分离效应,实现高浓度RSF水溶液的稳定自凝聚及微介观结构调控,并将多种几何尺寸的丝素纳米原纤(SFNF)作为RSF材料的增强体,利用静电纺丝法最终获得力学增强型自凝聚RSF微纳米纤维膜。结果表明:盐离子体系中,柠檬酸钠对RSF液-液相分离的诱导效果最强,RSF溶液的β-折叠结构含量由初始33.8%增加至51.1%,RSF溶液整体黏度提升,可纺性提高;SFNF有效改善了RSF微纳米纤维膜的力学性能,断裂伸长率从2.06%提升至3.54%,断裂强度从0.46 MPa提升至0.49 MPa;此外,纤维膜的溶血率为2.58%,具有良好的血液相容性,在创面敷料领域具有潜在的应用前景。Objective The dissolution of silk fibroin protein disrupts its multi-order structure,leading to a decline in the mechanical properties of fibers,which in turn limits the application of micro-nano silk fibroin fiber membranes.By reconstructing the micro-mesoscopic structure of regenerated silk fibroin(RSF),this research aims to enhance the mechanical properties of RSF materials,thereby expanding their potential applications in the biomedical field.Method In this study,we simulated the microenvironment within silkworm glands and induced liquid-liquid phase separation in the regenerated silk fibroin(RSF)solution through a salt ion system.Silk fibroin nanofibri-llars(SFNF)of various geometric dimensions were employed as reinforcements for the RSF material.By employing electrospinning,we fabricated mechanically enhanced,self-coagulating RSF micro-nano fiber membranes.Results Sodium citrate(Na 3Citrate)solution was found the optimal system for inducing self-coagulation of RSF aqueous solutions.When the concentration of Na 3Citrate exceeded 0.6 mol/L and the concentration of the RSF solution was above 2%,the RSF aqueous solution began to undergo self-coagulation.This process intensified with increasing sodium citrate concentration.However,when the concentrations of both Na 3Citrate and RSF were excessively high(i.e.,RSF above 16%,Na 3Citrate above 1.2 mol/L),the degree of self-coagulation became excessive,leading to the rapid formation of flaky precipitates within 20 minutes of solution preparation.With increasing concentration of Na 3Citrate,the entanglement of RSF macromolecular chains became more compact,leading to an increase in theβ-sheet structure of the RSF solution from the initial 33.8%to 51.1%.This enhancement in internal flow resistance resulted in increased viscosity of the RSF solution,thereby improving its spinnability.At a Na 3Citrate concentration of 1.0 mol/L,a voltage of 22 kV,a flow rate of 0.2 mL/h,and a spinning distance of 16 cm,the fiber diameter and coefficient of variation(CV)were minimized,sug

关 键 词：再生丝素蛋白 自凝聚 静电纺丝 微纳米纤维 力学增强 生物医用材料 

分 类 号：TS104.7[轻工技术与工程—纺织工程]