平安系列小麦品种(系)穗发芽抗性鉴定及基因的分子标记检测  

Identification of pre-harvest sprouting resistance and molecular marker detectionof genes in Ping′an series wheat varieties(lines)

作　　者：吕庆丰 朱昌涛 王刚 吕元丰 闫泽东 张珍 张立琳 LYU Qingfeng;ZHU Changtao;WANG Gang;LYU Yuanfeng;YAN Zedong;ZHANG Zhen;ZHANG Lilin(Henan Ping′an Seed Industry Co.,Ltd.,Wenxian 454850,China;Agricultural Technology Promotion Center of Xincai County,Xincai 463500,China;Service Center of Rural Industry Development in Henan Province,Zhengzhou 450000,China)

机构地区：[1]河南平安种业有限公司,河南温县454850 [2]新蔡县农业技术推广中心,河南新蔡463500 [3]河南省乡村产业发展服务中心,河南郑州450000

出　　处：《北方农业学报》2024年第6期13-21,共9页Journal of Northern Agriculture

基　　金：2023年中原学者工作站(234400510009)。

摘　　要：【目的】了解平安系列小麦品种(系)的穗发芽抗性及遗传组成。【方法】通过整穗发芽法对14个平安系列品种(系)的穗发芽特性进行分析,并用5个抗穗发芽基因的分子标记对穗发芽抗性基因来源进行检测。【结果】14个品种(系)中,平安11号相对穗发芽指数为0.19,达到抗穗发芽水平;平安658和平安701相对穗发芽指数分别为0.35和0.37,达到中抗穗发芽水平。抗穗发芽基因分子标记检测结果表明,TaPHS1基因在3个位点(-222、+646、+666)组成的单倍型相对穗发芽指数从低到高依次为CGAC<TGAC<TGAT<TATT,平安11号具有抗穗发芽能力最好的单倍型TaPHS1抗穗发芽基因,平安901等7个小麦品种(系)含有TaVp1B3抗穗发芽基因,平安12等4个小麦品种含有PM19A-1抗穗发芽基因,14个小麦品种(系)均不含Dorm-1和Tamyb10-D抗穗发芽基因。【结论】平安11号是唯一具有抗穗发芽单倍型CGAC的品种,可用于培育抗穗发芽小麦新品种和发掘抗穗发芽新基因。【Objective】To investigate the pre-harvest sprouting resistance and genetic composition of Ping′an series wheat varieties(lines).【Methods】The pre-harvest sprouting characteristics of 14 Ping′an series varieties(lines)were analyzed by whole ear germination method,and the sources of pre-harvest sprouting resistance genes were detected by molecular markers of 5 anti pre-harvest sprouting genes.【Results】Among the 14 varieties(lines),the relative pre-harvest sprouting index of Ping′an No.11 was 0.19,reached the level of resistance;the relative pre-harvest sprouting indexes of Ping′an 658 and Ping′an 701 were 0.35 and 0.37,respectively,reached the level of moderately resistance.The results of molecular marker detection of anti pre-harvest sprouting genes showed that the relative pre-harvest sprouting index of haplotype composed of three loci(-222、+646、+666)of TaPHS1 gene was CGAC<TGAC<TGAT<TATT from low to high,and Ping′an No.11 contained the best haplotype of TaPHS1 gene of anti pre-harvest sprouting,7 wheat varieties(lines)including Ping′an 901 contained anti pre-harvest sprouting gene TaVp1B3,4 wheat varieties including Ping′an 12 contain anti pre-harvest sprouting gene PM19A-1,all 14 wheat varieties(lines)contain neither of anti pre-harvest sprouting gene Dorm-1 or Tamyb10-D.【Conclusion】Ping′an No.11 is the only variety with hap-CGAC resistant to pre-harvest sprouting,which can be used in cultivating new varieties of anti pre-harvest sprouting wheat and discovering new anti pre-harvest sprouting gene.

关 键 词：小麦 穗发芽抗性 抗性鉴定 发芽指数 分子标记 等位变异 

分 类 号：S512.1[农业科学—作物学] Q75[生物学—分子生物学]