黄瓜枯萎病拮抗芽孢杆菌PSYC4的筛选及鉴定  

Screening and identification of antagonistic Bacillus PSYC4 from cucumber Fusarium wilt

作　　者：苏洁文 彭晨 管利 孙星 SU Jiewen;PENG Chen;GUAN Li;SUN Xing(College of Life Sciences,Anhui University,Hefei 230601,China;School of Biology and Food Engineering,Chuzhou University,Chuzhou 239099,China)

机构地区：[1]安徽大学生命科学学院,安徽合肥230601 [2]滁州学院生物与食品工程学院,安徽滁州239099

出　　处：《北方农业学报》2024年第6期39-46,共8页Journal of Northern Agriculture

基　　金：安徽省科技厅自然科学基金面上项目(2108085MC87)。

摘　　要：【目的】获得高效拮抗黄瓜枯萎病的生防菌。【方法】采用牛津杯法从黄海海域海水样品中筛选对黄瓜枯萎病病原菌具有较强拮抗作用的菌株,温室测定其对黄瓜种子发芽出苗的影响,并对影响最小的菌株进行革兰氏染色观察、生理生化鉴定、16S r DNA序列分析。【结果】共筛选出对黄瓜枯萎病病原菌具有较强拮抗作用的菌株6株,分别为PSYC4、PSYC22、PSYC24、PSYC161、PSYC162、PSYC132,其中菌株PSYC4对黄瓜种子发芽出苗影响最小,发芽率为91.67%,出苗率为100%。经鉴定,PSYC4为短小芽孢杆菌(Bacillus pumilus),对黄瓜专化型尖孢镰刀菌具有较强拮抗效果,其抑菌率为69.11%。【结论】菌株PSYC4能有效抑制黄瓜专化型尖孢镰刀菌,对黄瓜枯萎病具有显著的生防效果。【Objective】To obtain effective antagonistic biocontrol bacteria against cucumber Fusarium wilt.【Methods】The strains with stronger antagonistic effect to cucumber Fusarium wilt were screened from the samples of Yellow Sea by Oxford Cup method,the effect on germination and seedling of cucumber seeds was determined in greenhouse,the strain with the least influence by Gram staining observe,physiological and biochemical identification and 16S rDNA sequence analysis.【Results】A total of 6 strains with strong antagonistic effect against cucumber Fusarium wilt were screened,including PSYC4,PSYC22,PSYC24,PSYC161,PSYC162 and PSYC132.Among them,strain PSYC4 had the least effect on cucumber seed germination and seedling,with a germination rate of 91.67%and a seedling emergence rate of 100%.The strain PSYC4 was identified as Bacillus pumilus,which had a stronger antagonistic effect on Fusarium oxysporum f.sp.cucumerinum,with an inhibition rate of 69.11%.【Conclusion】Strain PSYC4 can effectively inhibit the Fusarium oxysporum f.sp.cucumerinum,and has a significant biocontrol effect on cucumber Fusarium wilt.

关 键 词：黄瓜枯萎病 海洋细菌 短小芽孢杆菌 黄瓜专化型尖孢镰刀菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.421.13[农业科学—植物保护]