变温条件和赤霉素处理对金莲花种子萌发的影响  

Effects of varied temperature conditions and gibberellin treatment on seed germinationof Trollius chinensis Bunge

作　　者：高新磊 刘利红 丁尚奇 王秀艳 刘凤玲 宋建垒 秦立金 王晓娟 GAO Xinlei;LIU Lihong;DING Shangqi;WANG Xiuyan;LIU Fengling;SONG Jianlei;QIN Lijin;WANG Xiaojuan(College of Agriculture,Chifeng University,Chifeng 024000,China;Xilin Gol Vocational College,Xilinhot 026000,China;Forest Defense Station of Forestry and Grassland Administration of Duolun County of Xilin Gol League in Inner Mongolia,Duolun 027300,China;Cultural Heritage Protection and Research Center of Xilin Gol League,Zhenglan Banner 027200,China;Grassland Workstation of Zhenglan Banner,Zhenglan Banner 027200,China)

机构地区：[1]赤峰学院农学院,内蒙古赤峰024000 [2]锡林郭勒职业学院,内蒙古锡林浩特026000 [3]内蒙古锡林郭勒盟多伦县林业和草原局森防站,内蒙古多伦027300 [4]锡林郭勒盟文化遗产保护研究中心,内蒙古正蓝旗027200 [5]正蓝旗草原工作站,内蒙古正蓝旗027200

出　　处：《北方农业学报》2024年第6期68-76,共9页Journal of Northern Agriculture

基　　金：锡林郭勒盟科技计划项目(202208)。

摘　　要：【目的】探究不同变温条件、赤霉素浓度、浸泡时间对金莲花种子萌发活力的影响,为金莲花种子萌发活力条件筛选及其播种提供参考。【方法】以2021年采集的金莲花种子作为试验材料,分别用不同浓度的赤霉素(0、100、250、500、800、1 000、1 500 mg/L)浸泡24、48 h,并模拟田间温度变化设置不同变温条件(15/5、20/10、25/15、25/20、30/20、30/25℃)进行发芽试验,对金莲花种子的萌发时间以及25/20℃变温条件下不同赤霉素浓度、浸泡时间和浸泡24 h处理下不同变温条件、赤霉素浓度的发芽率、发芽势、发芽指数进行差异分析。【结果】25/20℃变温条件下250 mg/L赤霉素浸泡24、48 h处理,30/25℃变温条件下250 mg/L赤霉素浸泡24 h处理和800、1 000 mg/L赤霉素浸泡48 h处理金莲花种子萌发开始时间较短,均为6 d。25/20℃变温条件下,500、800 mg/L赤霉素浸泡48 h处理金莲花种子发芽率、发芽势、发芽指数均显著高于浸泡24 h处理(P<0.05);在同一浸泡时间下,250、500 mg/L赤霉素处理种子发芽率、发芽势、发芽指数均较高。浸泡24 h处理下,20/10、25/15、25/20℃变温条件下,250 mg/L赤霉素处理的金莲花种子发芽率、发芽势、发芽指数均显著高于其他赤霉素浓度处理(P<0.05);250 mg/L赤霉素处理下,25/20℃变温条件下金莲花种子发芽率、发芽势和发芽指数均显著高于其他变温条件(P<0.05)。【结论】金莲花种子萌发对变温条件和赤霉素浓度处理的敏感性有所不同,过高或过低的温度以及赤霉素浓度处理均不利于金莲花种子的萌发;在浸泡24 h、25/20℃变温条件下,250 mg/L赤霉素处理能够更好地提升金莲花种子的萌发活力。【Objective】To investigate the effects of different varied temperature conditions,gibberellin concentrations,and soaking times on the germination activity of Trollius chinensis Bunge seeds,and to provide reference for the screening of germination activity conditions and sowing of T.chinensis Bunge seeds.【Methods】The seeds of T.chinensis Bunge collected in 2021 as experimental materials were respectively soaked with different concentrations of gibberellin(0,100,250,500,800,1000,1 500 mg/L) for 24 h and 48 h,and varied temperature conditions(15/5,20/10,25/15,25/20,30/20,30/25 ℃) were set to simulate field temperature changes for germination experiments. The germination time of the seeds of T. chinensis Bunge and the germination rate,germination potential,and germination index were analyzed under 25/20 ℃ varied temperature conditions in different gibberellin concentrations and soaking times and soaking for 24 h in different varied temperature conditions and gibberellin concentrations.【Results】The germination start time of T. chinensis Bunge seeds was shorter under the conditions of soaking in 250 mg/L gibberellin for 24 h and 48 h at 25/20 ℃,soaking in 250 mg/L gibberellin for 24 h at 30/25 ℃,and soaking in 800 and 1 000 mg/L gibberellin for 48 h at 30/25 ℃,all of which were 6 d. Under the varied temperature condition of 25/20 ℃,the germination rate,germination potential,and germination index of T. chinensis Bunge seeds soaked for 48 h in 500 and 800 mg/L gibberellin treatments were significantly higher than soaked for 24 h(P<0.05);at the same soaking time,seeds treated with 250 and 500 mg/L gibberellin showed higher germination rate,germination potential,and germination index. Under soaking for 24 h and varied temperature conditions of 20/10,25/15 and 25/20 ℃,the germination rate,germination potential,and germination index of T. chinensis Bunge seeds treated with 250 mg/L gibberellin were significantly higher than those treated with other gibberellin concentrations(P<0.05);under the treatme

关 键 词：金莲花 种子 赤霉素浓度 变温条件 浸泡时间 萌发活力 

分 类 号：S567.239[农业科学—中草药栽培] S482.8[农业科学—作物学]