Integrative Analysis of the Transcriptome and Metabolome of Poplar,Populus deltoides‘Shalinyang’.(Salicaceae),Reveals Responses to Attack by Anoplophora glabripennis(Coleoptera)  

作　　者：Jiayu Luo Jianfeng Liu Zuoxiang Sun Pengpeng Shao Danyang Wang Jianzhong Yao Binzhou Li Lijun Dong Dandan Cao Jianrong Wei 

机构地区：[1]College of Life Science,Institute of Life Science and Green Development,Hebei University,Baoding,071002,China [2]Experimental Center of Desert Forest,Chinese Academy of Forestry,Bayannur,015200,China [3]Shanxi Experiment Bureau for High-Yield Poplar Forests,Datong,037006,China [4]Hebei Innovation Center for Bioengineering and Biotechnology,Hebei University,Baoding,071002,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第9期2167-2188,共22页国际实验植物学杂志(英文)

基　　金：supported by the National Natural Science Foundation of China(Nos.32271891 and 32171798).

摘　　要：Anoplophora glabripennis is one of the most devastating wood-boring beetles that attacks poplars.However,one poplar species,Populus deltoides,has strong resistance to Anoplophora glabripennis infestation,the underlying defense mechanisms against Anoplophora glabripennis are poorly understood.Secondary metabolites play a crucial role in plants to combat biological stress.Here,based on transcriptome and metabolome,we demonstrated that the mechanisms for responses to mechanical damage and insect infestation were different.The degree of reactions to adult groove production,larval incubation,and larval frass production was not identical.In addition,the potential genes with insect resistance activity were identified.Predominant differentially expressed genes(DEGs)found in the phloem of Populus deltoides include anthocyanidin 3-O-glucosyltransferase5(PdUGT72E),peroxidase 73(PdPod73),peroxidase A2(PdPodA2)and macrophage migration inhibitory factor(PdMIF)responded to stress caused by Anoplophora glabripennis,which further resulted in activation of the plant defense system against insects via changes in regulation of metabolic pathways,such as tyrosine metabolism pathway,phenylpropanoid biosynthesis pathway and flavonoid biosynthesis pathway.Therefore,this work has laid a foundation for further unraveling the mechanisms involved in this interaction.

关 键 词：Populus deltoides‘Shalinyang’ Anoplophora glabripennis transcriptome and metabolome defense response 

分 类 号：F42[经济管理—产业经济]